CAJ | 학술논문

目的：研究活血化瘀法对大鼠心肌组织微环境中CD34、血管内皮生长因子(VEGF)的影响，探讨其治疗急性心肌梗死的机制。方法 Sprague-Dawley大鼠32只随机分为假手术组(n=8)、模型组(n=8)、中药+粒细胞集落刺激因子(G-CSF)组(n=8)和G-CSF组(n=8)。各组均于造模3 h后给予相应药物，连续6 d，第7天处死取材。苏木素-伊红(HE)染色观察病理形态，免疫组化染色检测梗死心肌边缘区CD34、VEGF、Ki-67阳性细胞表达。结果模型组、中药+G-CSF组和G-CSF组CD34、VEGF、Ki-67阳性细胞表达均高于假手术组(P<0.05)；中药+G-CSF组、G-CSF组高于模型组(P<0.05)；中药+G-CSF组CD34、VEGF阳性表达高于G-CSF组(P<0.05)，两组Ki-67阳性细胞表达无显著性差异(P>0.05)。结论活血化瘀法能够提高CD34和VEGF的表达，其作用优于单独使用G-CSF，可以有效治疗急性心肌梗死。
목적：연구활혈화어법대대서심기조직미배경중CD34、혈관내피생장인자(VEGF)적영향，탐토기치료급성심기경사적궤제。방법 Sprague-Dawley대서32지수궤분위가수술조(n=8)、모형조(n=8)、중약+립세포집락자격인자(G-CSF)조(n=8)화G-CSF조(n=8)。각조균우조모3 h후급여상응약물，련속6 d，제7천처사취재。소목소-이홍(HE)염색관찰병리형태，면역조화염색검측경사심기변연구CD34、VEGF、Ki-67양성세포표체。결과모형조、중약+G-CSF조화G-CSF조CD34、VEGF、Ki-67양성세포표체균고우가수술조(P<0.05)；중약+G-CSF조、G-CSF조고우모형조(P<0.05)；중약+G-CSF조CD34、VEGF양성표체고우G-CSF조(P<0.05)，량조Ki-67양성세포표체무현저성차이(P>0.05)。결론활혈화어법능구제고CD34화VEGF적표체，기작용우우단독사용G-CSF，가이유효치료급성심기경사。
Objective To explore the effect of Activating Blood to Resolve Stagnation on the expression of CD34 and vascular endotheli-al growth factor (VEGF) in rats with acute myocardial infarction. Methods 32 Sprague-Dawley rats were randomly divided into sham opera-tion group (A, n=8), model group (B, n=8), Xuesaitong Injection + granulocyte colony-stimulating factor (G-CSF) group (C, n=8) and G-CSF group (D, n=8). Corresponding medicine was given to each group 3 hours after modeling, for 6 days. Pathomorphological changes were observed through HE staining, and the expression of CD34, VEGF and Ki-67 were observed through immunohistochemical staining. Re-sults The expressions of CD34, VEGF and Ki-67 were higher in groups B, C and D than in group A (P<0.05), and were higher in group groups C and D than in group B (P<0.05). The expressions of CD34 and VEGF were higher in group C than in group D (P<0.05). However, there was no significant difference in the expression of Ki-67 between 2 groups (P>0.05). Conclusion The expression of CD34 and VEGF in-creases with Activating Blood to Resolve Stagnation method, which is superior to using G-CSF only. Activating Blood to Resolve Stagna-tion may play an important role in the treatment of acute myocardial infarction.