CAJ | 학술논문

目的：探讨灵芝多糖对荷膀胱癌T24细胞小鼠化疗后腹腔巨噬细胞免疫功能的影响。方法2012年5月—2014年10月建立荷膀胱癌T24细胞小鼠模型15只，采用随机数字表法分为3组：0.9％氯化钠溶液组、顺铂组、顺铂+灵芝多糖组，每组5只。分别以0.9％氯化钠溶液、0.9％氯化钠溶液、灵芝多糖灌胃18 d，以0.9％氯化钠溶液、顺铂、顺铂腹腔注射5d。收集小鼠腹腔巨噬细胞，采用流式细胞仪检测腹腔巨噬细胞对异硫氰酸荧光素-右旋糖酐（FITC-dextran）的吞噬能力，以FITC-dexran阳性率表示；采用酶联免疫吸附试验法（ELISA）检测腹腔巨噬细胞白介素1（IL-1）和肿瘤坏死因子α（TNF-α）表达水平；采用荧光定量PCR （RT-PCR）检测腹腔巨噬细胞IL-1 mRNA和TNF-αmRNA表达水平。结果顺铂+灵芝多糖组小鼠FITC-dextran阳性率较顺铂组升高4.5％；顺铂+灵芝多糖组小鼠CD11b -FITC阳性率和CD68-FITC阳性率分别较顺铂组升高11.5％和13.4％，但均未能达到0.9％氯化钠溶液组巨噬细胞中阳性率。3组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平比较，差异有统计学意义（ P＜0.05）；其中顺铂组和顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平较0.9％氯化钠溶液组降低（ P＜0.05）；顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平较顺铂组升高（ P＜0.05）。结论灵芝多糖能够提高荷膀胱癌T24细胞小鼠腹腔巨噬细胞的吞噬作用，从而提高机体的免疫功能，其机制可能与增强IL-1和TNF-α的表达水平有关。
목적：탐토령지다당대하방광암T24세포소서화료후복강거서세포면역공능적영향。방법2012년5월—2014년10월건립하방광암T24세포소서모형15지，채용수궤수자표법분위3조：0.9％록화납용액조、순박조、순박+령지다당조，매조5지。분별이0.9％록화납용액、0.9％록화납용액、령지다당관위18 d，이0.9％록화납용액、순박、순박복강주사5d。수집소서복강거서세포，채용류식세포의검측복강거서세포대이류청산형광소-우선당항（FITC-dextran）적탄서능력，이FITC-dexran양성솔표시；채용매련면역흡부시험법（ELISA）검측복강거서세포백개소1（IL-1）화종류배사인자α（TNF-α）표체수평；채용형광정량PCR （RT-PCR）검측복강거서세포IL-1 mRNA화TNF-αmRNA표체수평。결과순박+령지다당조소서FITC-dextran양성솔교순박조승고4.5％；순박+령지다당조소서CD11b -FITC양성솔화CD68-FITC양성솔분별교순박조승고11.5％화13.4％，단균미능체도0.9％록화납용액조거서세포중양성솔。3조소서복강거서세포IL-1、 TNF-α표체수평급IL-1 mRNA、 TNF-αmRNA표체수평비교，차이유통계학의의（ P＜0.05）；기중순박조화순박+령지다당조소서복강거서세포IL-1、 TNF-α표체수평급IL-1 mRNA、 TNF-αmRNA표체수평교0.9％록화납용액조강저（ P＜0.05）；순박+령지다당조소서복강거서세포IL-1、 TNF-α표체수평급IL-1 mRNA、 TNF-αmRNA표체수평교순박조승고（ P＜0.05）。결론령지다당능구제고하방광암T24세포소서복강거서세포적탄서작용，종이제고궤체적면역공능，기궤제가능여증강IL-1화TNF-α적표체수평유관。
Objective To explore the effects of ganoderma lucidum polysaccharides ( GLP) on the immune function of macrophages in mice bearing human ladder cancer T 24 cells.Methods Nude mice model bearing the human bladder cancer T 24 cells was established from May 2012 to October 2014, mice were divided into three groups by random number table method:0.9%sodium chloride solution group , cisplatin group and cisplatin +GLP group, there were 5 mice in each group.Mice in the above three groups received intragastric administration of 0.9% sodium chloride solution , 0.9% sodium chloride solution and GLP, respectively , and received intraperitoneal injection of 0.9% sodium chloride solution , cisplatin and cisplatin , respectively.The peritoneal macrophages were then collected , the phagocytic rate of peritoneal macrophages against FITC -dextran was further detected by flow cytometry , the levels of IL-1 and TNF-αin peritoneal macrophages were measured by ELISA, and real-time fluorescent quantitative polymerase chain reaction ( qPCR) was used to analyze the mRNA levels of IL-1 and TNF-αin peritoneal macrophages.Results The positive rates of FITC -dextran, CD11b -FITC and CD68 -FITC among mice in cisplatin+GLP group were 4.5%, 11.5%and 13.4%higher than those among mice in cisplatin group , respectively , the above indicators among mice in cisplatin +GLP group and cisplatin group were lower than those among mice in 0.9%sodium chloride solution group , respectively.There were significant differences in levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among three groups of mice ( P <0.05 ); levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among mice in cisplatin +GLP group and cisplatin group were significantly lower than those among mice in 0.9%sodium chloride solution group , respectively ( P<0.05); the above indicators among mice in cisplatin +GLP group were significantly higher than those among mice in cisplatin group ( P<0.05 ) .Conclusion GLP could improve the phagocytic function of peritoneal macrophages in mice , thus can improve immune function of body , the mechanism of which may be related to the enhancement effect of GLP on IL-1 and TNF-αexpression levels.