CAJ | 학술논문

目的：探讨 CD47、钙网织蛋白（CRT）在乳腺癌细胞凋亡过程中表达的变化和意义。方法用不同浓度的紫杉醇（0．1、1．0、10．0、100．0μmol／L）作用于 MDA-MB-231细胞。在不同的处理时间点（24、48、72、96 h），噻唑蓝比色法（MTT）检测细胞抑制率变化，流式细胞术测定 CD47、CRT 的表达。结果MDA-MB-231细胞的生长抑制率和紫杉醇浓度呈一定的剂量、时间相关性。各处理组在培养24 h 时 CD47的表达均显著高于对照组，其中10．0μmol／L 组表达高于1．0μmol／L 组（F ＝34．71，P ＜0．05）；各处理组在培养48 h 时 CD47的表达均显著高于对照组，其中10．0μmol／L 组表达高于1．0μmol／L 组和0．1μmol／L 组（F ＝24．272，P ＜0．05）；1．0μmol／L 组在培养72 h 时 CD47的表达高于对照组和10．0μmol／L 组（F ＝3．713，P1＝0．023，P2＝0．02，P ＜0．05）；0．1μmol／L 组在培养96 h 时 CD47的表达高于对照组、1．0μmol／L 组和10．0μmol／L 组（F ＝5449，P1＝0．022，P2＝0．009，P3＝0．008）。各处理组在培养24 h 时 CRT 的表达均显著高于对照组，其中0．1μmol／L 组和1．0μmol／L 组的表达又高于10．0μmol／L 组（F ＝48．007，P ＜0．05）；各处理组在培养48 h 时 CRT 的表达均显著高于对照组，其中0．1μmol／L 组和1．0μmol／L 组的表达又高于10．0μmol／L 组（F ＝98．683，P ＜0．05）。结论CD47和 CRT 随着乳腺癌细胞发生凋亡其表达相应增加，和细胞抑制率存在一定的相关性。
목적：탐토 CD47、개망직단백（CRT）재유선암세포조망과정중표체적변화화의의。방법용불동농도적자삼순（0．1、1．0、10．0、100．0μmol／L）작용우 MDA-MB-231세포。재불동적처리시간점（24、48、72、96 h），새서람비색법（MTT）검측세포억제솔변화，류식세포술측정 CD47、CRT 적표체。결과MDA-MB-231세포적생장억제솔화자삼순농도정일정적제량、시간상관성。각처리조재배양24 h 시 CD47적표체균현저고우대조조，기중10．0μmol／L 조표체고우1．0μmol／L 조（F ＝34．71，P ＜0．05）；각처리조재배양48 h 시 CD47적표체균현저고우대조조，기중10．0μmol／L 조표체고우1．0μmol／L 조화0．1μmol／L 조（F ＝24．272，P ＜0．05）；1．0μmol／L 조재배양72 h 시 CD47적표체고우대조조화10．0μmol／L 조（F ＝3．713，P1＝0．023，P2＝0．02，P ＜0．05）；0．1μmol／L 조재배양96 h 시 CD47적표체고우대조조、1．0μmol／L 조화10．0μmol／L 조（F ＝5449，P1＝0．022，P2＝0．009，P3＝0．008）。각처리조재배양24 h 시 CRT 적표체균현저고우대조조，기중0．1μmol／L 조화1．0μmol／L 조적표체우고우10．0μmol／L 조（F ＝48．007，P ＜0．05）；각처리조재배양48 h 시 CRT 적표체균현저고우대조조，기중0．1μmol／L 조화1．0μmol／L 조적표체우고우10．0μmol／L 조（F ＝98．683，P ＜0．05）。결론CD47화 CRT 수착유선암세포발생조망기표체상응증가，화세포억제솔존재일정적상관성。
Objective To investigate the CD47 and calreticulin(CRT)expression in the apoptosis of breast cancer cells.Methods Breast cancer cell line MDA-MB-231 was treated with paclitaxel at the dose of 0.1,1.0, 10.0 and 100.0 μmol/L.The inhibitory effect was detected by MTT method at the four time points(24,48,72 and 96 hours),and the CD47 and CRT expression were measured by flow cytometry.Results The inhibitory rates of MDA-MB-231 cells was correlative with Taxol dose.Compared with those in the control group,the CD47 expression in the treatment groups was significantly increased at 24 hour,and in the 10.0 μmol/L treatment group,itwas higher than that in the 1.0 μmol/L treatment group(F =34.71,P <0.05).Compared with those in the control group,the CD47 expression in all treatment groups was significantly elevated at 48 hour,and 10.0 μmol/L treatment group was more higher than 1.0 μmol/L and 0.1 μmol/L treatment groups(F =24.272,P <0.05).The CD47 expression in the 1.0 μmol/L treatment group was more higher than that in the control group and the10.0 μmol/L treatment group at 72 hours(F =3.713,P1 =0.023,P2 =0.02,P <0.05).The CD47 expression in the 0.1 μmol/L treatment group was more higher than that in the control group,1.0 μmol/L and 10.0 μmol/L treatment groups at 96 hours(F =5449,P1 =0.022,P2 =0.009,P2 =0.008,P <0.05).Compared with those in the control group,the CRT expression in all treatment groups was significantly elevated at 24 hour,and in the 0.1 μmol/L and 1.0 μmol/L treatment groups,it were higher than that in the10.0 μmol/L treatment group(F =48.007,P <0.05).Compared with those in the control group,the CRT expression in all treatment groups was significantly elevated at 48 hour,and in the 0.1 μmol/L and 1.0 μmol/L treatment groups,itwere higher than that in the10.0 μmol/L treatment group(F =98.683,P <0.05).Conclusions The expression of CD47 and CRT are increased in the apoptosis of breast cancer cells,which are correlated with inhibitory rates of MDA-MB-231 cells.