CAJ | 학술논문

目的：探讨基质细胞衍生因子-1（SDF-1）及 Wnt 信号通路对诱导生成的肌成纤维细胞的生物学特性的影响。方法首先体外诱导骨髓间充质干细胞（BMSCs）分化为肌成纤维细胞（MF），然后改变SDF-1及（或）Wnt 信号，通过划痕实验观察 MF 迁移能力的变化，qRT-PCr 检测Ⅰ型胶原（ColⅠ）、α-平滑肌肌动蛋白（α-SMA）表达的改变。结果转化生长因子（TGF-β）及高浓度血清等条件的诱导，可使 BMSCs 分化为 MF。阻断 SDF-1或 Wnt 信号时，会显著抑制 MF 的迁移及 Col-Ⅰ的分泌，阻断 Wnt 信号可明显抑制 BMSCs向 MF 的分化。结论SDF-1与 Wnt 信号参与调节 MF 的迁移及 ColⅠ分泌，Wnt 信号对 BMSCs 向 MF 分化具有重要作用。
목적：탐토기질세포연생인자-1（SDF-1）급 Wnt 신호통로대유도생성적기성섬유세포적생물학특성적영향。방법수선체외유도골수간충질간세포（BMSCs）분화위기성섬유세포（MF），연후개변SDF-1급（혹）Wnt 신호，통과화흔실험관찰 MF 천이능력적변화，qRT-PCr 검측Ⅰ형효원（ColⅠ）、α-평활기기동단백（α-SMA）표체적개변。결과전화생장인자（TGF-β）급고농도혈청등조건적유도，가사 BMSCs 분화위 MF。조단 SDF-1혹 Wnt 신호시，회현저억제 MF 적천이급 Col-Ⅰ적분비，조단 Wnt 신호가명현억제 BMSCs향 MF 적분화。결론SDF-1여 Wnt 신호삼여조절 MF 적천이급 ColⅠ분비，Wnt 신호대 BMSCs 향 MF 분화구유중요작용。
Objective To explore the impact of SDF-1 and wnt signal pathway on the biological characteris-tics of Myofibroblast .Methods Firstly the BMSCs were induced to differentiate into MF,then after the change of SDF-1 and (or)Wnt signal pathway ,the MF's type Ⅰ collagen(ColⅠ)and α-smooth muscle actin(α-SMA)mRNA expression were detected and the migration ability of MF was evaluated by scratch experiments.Results (1)BM-SCs could be induced to differentiation into MF in the condition of culture medium including transforming growth fac-tor-β(TGF-β)and high concentration of serum in vitro;(2)When SDF-1 /CXCR4 or Wnt signal pathway were blocked ,the migration ability of MF and secretion of ColⅠwas inhibited significantly;(3)The BMSCs differentiation into MF can be inhibited by blocking Wnt signal pathway.Conclusion SDF-1 and Wnt signal pathway play an im-portant role in the regulation of MF migrationand ColⅠ secretion ,and Wnt signal pathway involves in the regulation of BMSCs differentiation into MF.