医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
31期
32-34
,共3页
运动性猝死%基因多态性%等位基因特异性PCR
運動性猝死%基因多態性%等位基因特異性PCR
운동성졸사%기인다태성%등위기인특이성PCR
Exercise sudden death%Genetic polymorphism%Al ele specific PCR
目的建立一种中国人群运动性猝死相关基因多态性速测方法。方法用Chelex法提取外周静脉血基因组DNA，应用定点突变技术获得运动性猝死相关基因的突变阳性DNA模板，然后通过等位基因特异性(al ele-specific, AS)PCR法扩增目的基因。优化内参引物、多态引物的Tm值，引物浓度比例，PCR循环数等扩增条件；琼脂糖凝胶电泳进行基因分型。结果 AS-PCR可检测运动性猝死相关基因多态性，PCR产物琼脂糖凝胶分型与DNA测序结果完全一致。结论本研究建立的AS-PCR方法适用于中国人群运动性猝死相关基因的快速检测。
目的建立一種中國人群運動性猝死相關基因多態性速測方法。方法用Chelex法提取外週靜脈血基因組DNA，應用定點突變技術穫得運動性猝死相關基因的突變暘性DNA模闆，然後通過等位基因特異性(al ele-specific, AS)PCR法擴增目的基因。優化內參引物、多態引物的Tm值，引物濃度比例，PCR循環數等擴增條件；瓊脂糖凝膠電泳進行基因分型。結果 AS-PCR可檢測運動性猝死相關基因多態性，PCR產物瓊脂糖凝膠分型與DNA測序結果完全一緻。結論本研究建立的AS-PCR方法適用于中國人群運動性猝死相關基因的快速檢測。
목적건립일충중국인군운동성졸사상관기인다태성속측방법。방법용Chelex법제취외주정맥혈기인조DNA，응용정점돌변기술획득운동성졸사상관기인적돌변양성DNA모판，연후통과등위기인특이성(al ele-specific, AS)PCR법확증목적기인。우화내삼인물、다태인물적Tm치，인물농도비례，PCR순배수등확증조건；경지당응효전영진행기인분형。결과 AS-PCR가검측운동성졸사상관기인다태성，PCR산물경지당응효분형여DNA측서결과완전일치。결론본연구건립적AS-PCR방법괄용우중국인군운동성졸사상관기인적쾌속검측。
Objective To establish a simple method for testing exercise sudden death gene of Chinese. Methods Firstly, extract genomic DNA from peripheral venous blood. Secondly, use fixed point mutation technique to get the positive template, and then use the al ele specific (al ele specific, AS) PCR to amply the target gene. Thirdly, optimize the Tm value and concentration ratio of primers, PCR cycle number and a series of amplification conditions. Final y, analyze the PCR product by agarose gel electrophoresis and sequencing. Results The AS-PCR can detect sudden cardiac death gene polymorphism, the agarose gel electrophoresis genotyping results of PCR product coincide exactly with the result of DNA sequencing .Conclusion This new method established and optimized can be used for rapid detection of exercise sudden death related genes.
