CAJ | 학술논문

目的：探讨人参皂苷对人甲状腺癌 SW579细胞的增殖抑制作用及 C-myc、Bcl-2蛋白表达的影响。方法体外甲状腺癌 SW579细胞常规培养，实验分为对照组和人参皂苷组（20、40、80μg／mL），用 MTT法检测人参皂苷对细胞增殖抑制情况，Western-blot 法检测各组 SW 细胞的 C-myc 和 Bcl-2蛋白表达。结果20μg／mL、40μg／mL、80μg／mL 浓度人参皂苷对甲状腺癌 SW579细胞的抑制率分别为22．35％、51.76％、68.24％，与对照组相比具有明显的抑制作用（P ＜0．01），且随溶度增加，抑制作用增强（P ＜0.01）；免疫印迹检测显示：随着人参皂苷浓度增大，甲状腺癌 SW579细胞 C-myc 和 Bcl-2蛋白的表达水平均显著逐渐下降（P ＜0．01）。结论人参皂苷对人甲状腺癌 SW579细胞的增殖具有显著抑制作用，其作用可能与下调 C-myc和 Bcl-2蛋白表达相关。
목적：탐토인삼조감대인갑상선암 SW579세포적증식억제작용급 C-myc、Bcl-2단백표체적영향。방법체외갑상선암 SW579세포상규배양，실험분위대조조화인삼조감조（20、40、80μg／mL），용 MTT법검측인삼조감대세포증식억제정황，Western-blot 법검측각조 SW 세포적 C-myc 화 Bcl-2단백표체。결과20μg／mL、40μg／mL、80μg／mL 농도인삼조감대갑상선암 SW579세포적억제솔분별위22．35％、51.76％、68.24％，여대조조상비구유명현적억제작용（P ＜0．01），차수용도증가，억제작용증강（P ＜0.01）；면역인적검측현시：수착인삼조감농도증대，갑상선암 SW579세포 C-myc 화 Bcl-2단백적표체수평균현저축점하강（P ＜0．01）。결론인삼조감대인갑상선암 SW579세포적증식구유현저억제작용，기작용가능여하조 C-myc화 Bcl-2단백표체상관。
Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.