bcl-2小分子干扰 RNA 对人子宫内膜癌RL -952细胞阿霉素敏感性的影响
bcl-2소분자간우 RNA 대인자궁내막암RL -952세포아매소민감성적영향
Influence of bcl-2 Small Interference RNA on the Sensibility of Endometrial Carcinoma RL-952 Cells to Doxorubicin
  • 万方数据
    中国全科医学 중국전과의학
    CHINESE GENERAL PRACTICE
    2015年 24期 2917-2921 ,共5页

    周夫群%黄章骞%何尧

    주부군%황장건%하요

    子宫内膜肿瘤%基因, bcl-2%RNA, 小分子干扰%阿霉素%半胱氨酸蛋白酶类 子宮內膜腫瘤%基因, bcl-2%RNA, 小分子榦擾%阿黴素%半胱氨痠蛋白酶類
    자궁내막종류%기인, bcl-2%RNA, 소분자간우%아매소%반광안산단백매류
    Endometrial neoplasms%Genes,bcl-2%RNA,small interfering%Doxorubicin%Cysteine proteases
    目的：研究靶向bcl-2小分子干扰 RNA （ siRNA ）干扰序列对人子宫内膜癌RL-952细胞阿霉素（ DOX）敏感性的影响及相关作用机制。方法2014年5—8月，将siRNA干扰序列转染人子宫内膜癌RL-952细胞，设3个实验组：空白对照组、 bcl-2 siRNA干扰组（转染bcl-2 siRNA干扰序列）及阴性对照组（转染siRNA空载序列），检测bcl-2 siRNA干扰组的转染效率和3组bcl-2表达水平。将细胞随机分成5组，分别为空白对照组、阴性对照组（转染siRNA空载序列）、干扰组（转染bcl-2 siRNA干扰序列）、 DOX组（加入5μg／ml DOX）、联合组（转染siRNA干扰序列并加入5μg／ml DOX），检测细胞活力、细胞凋亡率、半胱氨酸蛋白酶（ Caspase ）-3、 Caspase-9活性及细胞色素C水平。结果 siRNA干扰序列转染人子宫内膜癌RL-952细胞的转染效率为（94.6±12.5）％。3组bcl-2表达水平比较，差异有统计学意义（F＝8.75， P＜0.05）。 bcl-2 siRNA干扰组bcl-2表达水平低于空白对照组和阴性对照组（q＝3.99、2.87， P＜0.01）。5组细胞活力比较，差异有统计学意义（F＝42.52， P＜0.05）。干扰组、DOX组、联合组细胞活力低于空白对照组（ q ＝7.66、8.64、6.89， P＜0.05）；联合组细胞活力低于DOX 组（ q＝6.76， P＜0.01）。5组细胞凋亡率比较，差异有统计学意义（F＝112.34， P＜0.05）。干扰组、 DOX组、联合组细胞凋亡率高于空白对照组（q＝5.78、4.99、6.21， P＜0.05）；联合组细胞凋亡率高于DOX组（q＝4.89， P＜0.01）。5组Caspase-3、 Caspase-9活性及细胞色素C水平比较，差异有统计学意义（ P＜0.05）。干扰组、 DOX组、联合组Caspase-3、 Caspase-9活性及细胞色素C水平高于空白对照组（P＜0.05）； DOX组、联合组Caspase-3活性高于阴性对照组，干扰组、 DOX组、联合组Caspase-9活性高于阴性对照组（ P＜0.05）；联合组Caspase-3活性高于干扰组， DOX组、联合组Caspase-9活性高于干扰组（ P＜0.05）；联合组Caspase-3、 Caspase-9活性及细胞色素C水平高于DOX组（ P＜0.05）。结论靶向bcl-2 siRNA干扰序列可以增强人子宫内膜癌RL-952细胞的DOX敏感性， bcl-2可作为人子宫内膜癌基因治疗的候选靶点。
    목적：연구파향bcl-2소분자간우 RNA （ siRNA ）간우서렬대인자궁내막암RL-952세포아매소（ DOX）민감성적영향급상관작용궤제。방법2014년5—8월，장siRNA간우서렬전염인자궁내막암RL-952세포，설3개실험조：공백대조조、 bcl-2 siRNA간우조（전염bcl-2 siRNA간우서렬）급음성대조조（전염siRNA공재서렬），검측bcl-2 siRNA간우조적전염효솔화3조bcl-2표체수평。장세포수궤분성5조，분별위공백대조조、음성대조조（전염siRNA공재서렬）、간우조（전염bcl-2 siRNA간우서렬）、 DOX조（가입5μg／ml DOX）、연합조（전염siRNA간우서렬병가입5μg／ml DOX），검측세포활력、세포조망솔、반광안산단백매（ Caspase ）-3、 Caspase-9활성급세포색소C수평。결과 siRNA간우서렬전염인자궁내막암RL-952세포적전염효솔위（94.6±12.5）％。3조bcl-2표체수평비교，차이유통계학의의（F＝8.75， P＜0.05）。 bcl-2 siRNA간우조bcl-2표체수평저우공백대조조화음성대조조（q＝3.99、2.87， P＜0.01）。5조세포활력비교，차이유통계학의의（F＝42.52， P＜0.05）。간우조、DOX조、연합조세포활력저우공백대조조（ q ＝7.66、8.64、6.89， P＜0.05）；연합조세포활력저우DOX 조（ q＝6.76， P＜0.01）。5조세포조망솔비교，차이유통계학의의（F＝112.34， P＜0.05）。간우조、 DOX조、연합조세포조망솔고우공백대조조（q＝5.78、4.99、6.21， P＜0.05）；연합조세포조망솔고우DOX조（q＝4.89， P＜0.01）。5조Caspase-3、 Caspase-9활성급세포색소C수평비교，차이유통계학의의（ P＜0.05）。간우조、 DOX조、연합조Caspase-3、 Caspase-9활성급세포색소C수평고우공백대조조（P＜0.05）； DOX조、연합조Caspase-3활성고우음성대조조，간우조、 DOX조、연합조Caspase-9활성고우음성대조조（ P＜0.05）；연합조Caspase-3활성고우간우조， DOX조、연합조Caspase-9활성고우간우조（ P＜0.05）；연합조Caspase-3、 Caspase-9활성급세포색소C수평고우DOX조（ P＜0.05）。결론파향bcl-2 siRNA간우서렬가이증강인자궁내막암RL-952세포적DOX민감성， bcl-2가작위인자궁내막암기인치료적후선파점。
    Objective To research the influence of bcl-2 targeting siRNA on the sensibility of endometrial carcinoma RL-952 cells to doxorubicin (DOX) .Methods From May to August in 2014, siRNA interference sequence were transfected into endometrial carcinoma RL-952 cells.The RL-952 cells were divided into three trial groups: blank control group , bcl-2 siRNA interference group ( with transfected bcl-2 siRNA interference sequence ) and negative control group ( with transfected siRNA empty sequence ) .The transfection efficiency of bcl-2 siRNA interference group and the protein expression level of bcl-2 of the three groups were tested .The RL-952 cells were divided into five groups: control blank group , negative control group ( with transfected siRNA empty sequence ) , interference group ( with transfected bcl-2 siRNA interference sequence ) , DOC group ( added with 5μg/ml DOX) , and combination group ( with transfected siRNA interference sequence and&nbsp;5 μg/ml DOX) .Cell viability, cell apoptosis rate, the activity of Caspase-3 and Caspase-9 and cytochrome C level of the five groups were tested.Results The efficiency of transfection from siRNA interference sequence to endometrial carcinoma RL-952 cells was (94.6 ±12.5)%.The three trial groups were significantly different in the protein expression level of bcl-2 (F=8.75, P<0.05) .The bcl-2 siRNA interference group was lower than blank control group and negative control group in the protein expression level ( q =3.99, 2.87; P <0.01 ) .The five groups were significantly different in cell viability ( F=42.52, P <0.05) .Interference group, DOC group and combination group were lower than blank control group in cell viability (q =7.66, 8.64, 6.89; P<0.05); combination group was lower than DOX group in cell viability (q=6.76, P<0.01).The five groups were significantly different in cell apoptosis rate (F=112.34, P<0.05) .Interference group, DOX group and combination group were higher than blank control group in cell apoptosis rate (q=5.78, 4.99, 6.21; P<0.05);combination group was higher than DOX group in cell apoptosis rate ( q=4.89, P<0.01) .The five groups were significantly different in the activity of Caspase-3 and Caspase-9 and cytochrome C level (P<0.05) .Interference group, DOX group and combination group were higher than the blank control group in the activity of Caspase-3 and Caspase-9 and cytochrome C level (P<0.05); DOX group, and combination group were higher than negative control group in the activity of Caspase-3 (P<0.05), and interference group, DOX group and combination group were higher than negative control gorup in the activity of Caspase-9 (P<0.05); combination group was higher than interference group in the activity of Caspase-3 (P<0.05), and DOX group and combination group were higher than interference group in the activity of Caspase-9 ( P<0.05 ); combination group was higher than DOX group in the activity of Caspase-3 and Caspase-9 and cytochrome C level (P<0.05) .Conclusion <br> bcl-2 targeting siRNA interference sequence can increase the sensitivity of endometrial carcinoma RL-952 cells to DOX. bcl-2 gene may be a potential target in the gene therapy of endometrial carcinoma .
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