CAJ | 학술논문

目的观察高湿环境下大鼠肝脏组织损伤及细胞凋亡情况，探讨高湿环境对大鼠肝脏的影响。方法32只SD大鼠随机分为两组，高湿20d组与对照组，每组16只大鼠。采用人工气候箱模拟高湿环境，条件设置为温度25℃、相对湿度（90%±2%）RH。高湿20d组大鼠每天置于气候箱中10h，其余时间与对照组饲养于相同环境，温度25℃，相对湿度（45%~50%）RH，时间为20d。动物模型制作完成后，取肝脏组织行免疫组化及Western实验观察两组肝脏细胞凋亡情况。结果高湿组Tunel法原位标记可观察到肝脏细胞凋亡，同时活化的Caspase-3酶表达显著升高。结论高湿环境可诱导大鼠肝脏细胞凋亡的发生，凋亡的关键酶Caspase-3在其中扮演了重要角色，为下一步研究高湿诱导细胞凋亡的具体机制提供了实验基础。
목적관찰고습배경하대서간장조직손상급세포조망정황，탐토고습배경대대서간장적영향。방법32지SD대서수궤분위량조，고습20d조여대조조，매조16지대서。채용인공기후상모의고습배경，조건설치위온도25℃、상대습도（90%±2%）RH。고습20d조대서매천치우기후상중10h，기여시간여대조조사양우상동배경，온도25℃，상대습도（45%~50%）RH，시간위20d。동물모형제작완성후，취간장조직행면역조화급Western실험관찰량조간장세포조망정황。결과고습조Tunel법원위표기가관찰도간장세포조망，동시활화적Caspase-3매표체현저승고。결론고습배경가유도대서간장세포조망적발생，조망적관건매Caspase-3재기중분연료중요각색，위하일보연구고습유도세포조망적구체궤제제공료실험기출。
Objective To observe the damage of rat liver tissue and the apoptosis of rat liver cells in high humidity environment so as to discuss the influence of high humidity on rat liver. Methods 32 SD rats were randomly divided into 2 groups:high humidity group and control group, 16 in each; artificial climate box was applied in imitating the high humidity environment, with the temperature of 25℃and relative humidity of 90±2%RH;rats in both groups were fed in the same environment with the temperature of 25℃and rela-tive humidity of 45%-50%RH for 20 days, during which, rats in high humidity group were kept in the artificial climate box for 10 hours every day; immunohistochemical study and Western Bolt were applied in observing the apoptosis of liver cells in rats in both groups. Results Apoptosis of rat liver cells in high humidity group was observed by TUNEL and the expression of activated enzyme Caspase-3 was obviously high. Conclusions High humidity environment may induce the apoptosis of rat liver cells and Caspase-3 as the key enzyme plays an important role in the process, which provides an experimental basis for further research on the mechanism of apop-tosis induced in high humidity environment.