世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2015年
5期
1108-1112
,共5页
孙美利%李蕾%王和宇%张晶%徐暾海%刘铜华
孫美利%李蕾%王和宇%張晶%徐暾海%劉銅華
손미리%리뢰%왕화우%장정%서돈해%류동화
斑鸠菊%总黄酮%正交试验%分光光度法
斑鳩菊%總黃酮%正交試驗%分光光度法
반구국%총황동%정교시험%분광광도법
Vernonia esculenta Hemsl.%total flavonoids%orthogonal array%spectrophotometry
目的：优选斑鸠菊果实中总黄酮的最佳提取工艺。方法：采用分光光度法，通过单因素实验考察乙醇浓度、提取次数、提取时间以及液料比对斑鸠菊果实中总黄酮提取率的影响。结果：最佳提取条件为乙醇浓度70%、提取4次，每次1 h、料液比为1：20。结论：采用最佳提取条件，得到的斑鸠菊果实中总黄酮含量为103.55 mg·g-1。
目的：優選斑鳩菊果實中總黃酮的最佳提取工藝。方法：採用分光光度法，通過單因素實驗攷察乙醇濃度、提取次數、提取時間以及液料比對斑鳩菊果實中總黃酮提取率的影響。結果：最佳提取條件為乙醇濃度70%、提取4次，每次1 h、料液比為1：20。結論：採用最佳提取條件，得到的斑鳩菊果實中總黃酮含量為103.55 mg·g-1。
목적：우선반구국과실중총황동적최가제취공예。방법：채용분광광도법，통과단인소실험고찰을순농도、제취차수、제취시간이급액료비대반구국과실중총황동제취솔적영향。결과：최가제취조건위을순농도70%、제취4차，매차1 h、료액비위1：20。결론：채용최가제취조건，득도적반구국과실중총황동함량위103.55 mg·g-1。
Spectrophotometry and single factor test were used in the investigation of effects on extraction results of total flavonoids from the fruit ofVernonia esculenta Hemsl. from aspects of ethanol concentration, frequency of extraction, length of extraction time, and the solid-liquid ratio. The extraction conditions were optimized by orthogonal test. The results showed that the optimum extraction conditions were ethanol concentration of 70%, extracted for 4 times, 1 h for each time, and the solid-liquid ratio of 1:20. Under these extraction conditions, the content of total flavonoids in the fruit ofVernonia esculenta Hemsl. was 103.55 mg·g-1.
