CAJ | 학술논문

目的：观察桂枝茯苓胶囊主要成分含量变化对痛经模型大鼠、盆腔炎模型大鼠、子宫肌瘤模型大鼠的影响。方法：采用分子烙印技术定量逐级敲除桂枝茯苓胶囊中主要成分（敲除比例为10%、20%、30%、40%、50%、60%、70%、80%、90%），分别观察对缩宫素所致大鼠痛经模型的扭体次数、潜伏期、子宫组织中ET-1及PGF2α的影响；对混合菌液加机械损伤造成的慢性盆腔炎模型大鼠的TNF-α、IL-2的影响；对雌激素负荷法所致大鼠子宫肌瘤模型的子宫重量、子宫系数及血清中雌二醇（E2）、孕酮（P）水平的影响。结果：逐级敲除15个主要成分后药效逐渐降低，敲除10%-40%时，与模型比较仍有显著差异（P<0.01），而敲除50%-90%时，与模型组无明显差异（P＞0.05），且药效比桂枝茯苓胶囊组明显降低（P<0.05或P<0.01）。结论：没食子酸、芍药苷、丹皮酚等15个化合物可能是桂枝茯苓胶囊发挥治疗作用的主要效应物质基础，同时也为桂枝茯苓胶囊主要成分的质控含量范围的制定提供了有益参考。
목적：관찰계지복령효낭주요성분함량변화대통경모형대서、분강염모형대서、자궁기류모형대서적영향。방법：채용분자락인기술정량축급고제계지복령효낭중주요성분（고제비례위10%、20%、30%、40%、50%、60%、70%、80%、90%），분별관찰대축궁소소치대서통경모형적뉴체차수、잠복기、자궁조직중ET-1급PGF2α적영향；대혼합균액가궤계손상조성적만성분강염모형대서적TNF-α、IL-2적영향；대자격소부하법소치대서자궁기류모형적자궁중량、자궁계수급혈청중자이순（E2）、잉동（P）수평적영향。결과：축급고제15개주요성분후약효축점강저，고제10%-40%시，여모형비교잉유현저차이（P<0.01），이고제50%-90%시，여모형조무명현차이（P＞0.05），차약효비계지복령효낭조명현강저（P<0.05혹P<0.01）。결론：몰식자산、작약감、단피분등15개화합물가능시계지복령효낭발휘치료작용적주요효응물질기출，동시야위계지복령효낭주요성분적질공함량범위적제정제공료유익삼고。
The rat model of dysmenorrhea, pelvic inflammation disease (PID), and uterine myoma were established to observe the effect ofGui-Zhi Fu-Ling Capsule (GZFLC) with the changes of main components. The molecular imprinted technology was used to quantitatively knock out main components in GZFLC (the knocked-out ratios were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%). And the observation was made on the writhing times, latency, and effects on ET-1 and PGF2α of uterine tissues in the pitocin-induced dysmenorrhea rat model. Effects on TNF-α and IL-2 were observed in PID rat model induced by mixed bacteria plus mechanical damages. Effects on the weight and index of uterus, levels of E2 and P in serum were detected on the uterine myoma rat model induced by estrogen-burden. The results showed that the GZFLC efficacy was decreased with gradient knockout of 15 main components. When 10%-40% was knocked out, there was still significant difference compared to the model group (P < 0.01). However, when 50%-90% was knocked out, there was no significant difference compared to the model group (P > 0.05). Meanwhile, the GZFLC efficacy was decreased significantly compared to the GZFLC group (P <0.05, orP < 0.01). It was concluded that 15 chemical compounds, which included gallic acid, paeoniflorin, paeonol, and etc., may be the main material basis of GZFLC. Meanwhile, it provided a useful reference for the quality control of main components in GZFLC.