CAJ | 학술논문

目的：研究放射创伤强度与主要组织相容性复合体缺失相关的自身免疫移植物抗宿主病的关系。方法受鼠予不同的全身放射强度进行全身放射，放射后回输T淋巴细胞去除的骨髓细胞。将受鼠分两组：①WT组：回输来自野生型c57Bl/6（H2b）细胞106/只；②mHc-/-组：回输来自主要组织相容性复合体（major histocompatibility complex，mHc）Ⅱ类分子缺失的c57Bl/6（H2b）细胞106/只。移植后记录小鼠体重、观察移植小鼠gVHd典型症状及生存率等；gVHd发病小鼠行病理切片确诊。结果①1000rad的全身放射强度剂量可以在异基因mHc全不相合移植（H2b→H2d）动物模型中诱导出的mHcⅡ类分子缺失相关的自身免疫gVHd。②1000rad的放射强度，mHc-/-组小鼠移植后体重下降明显、gVHd评分高、组织病理见大量的淋巴细胞浸润(P<0.05)。③减弱为800rad的放射强度两组小鼠生存率及体重无统计学差别，未见明显弓背、脱毛、腹泻、脱皮等gVHd症状（P>0.05）。结论主要组织相容性复合体缺失相关的自身免疫移植物抗宿主病与放射创伤强度相关
목적：연구방사창상강도여주요조직상용성복합체결실상관적자신면역이식물항숙주병적관계。방법수서여불동적전신방사강도진행전신방사，방사후회수T림파세포거제적골수세포。장수서분량조：①WT조：회수래자야생형c57Bl/6（H2b）세포106/지；②mHc-/-조：회수래자주요조직상용성복합체（major histocompatibility complex，mHc）Ⅱ류분자결실적c57Bl/6（H2b）세포106/지。이식후기록소서체중、관찰이식소서gVHd전형증상급생존솔등；gVHd발병소서행병리절편학진。결과①1000rad적전신방사강도제량가이재이기인mHc전불상합이식（H2b→H2d）동물모형중유도출적mHcⅡ류분자결실상관적자신면역gVHd。②1000rad적방사강도，mHc-/-조소서이식후체중하강명현、gVHd평분고、조직병리견대량적림파세포침윤(P<0.05)。③감약위800rad적방사강도량조소서생존솔급체중무통계학차별，미견명현궁배、탈모、복사、탈피등gVHd증상（P>0.05）。결론주요조직상용성복합체결실상관적자신면역이식물항숙주병여방사창상강도상관
Objective To explore the relationship between radiation dose and lack of major histocompatibility complex induced autoimmune graft-versus-host disease(gVHd).Method mice were conditioned with different doses of total body irradiation(TBi) and transplanted with T-cell depleted(Tcd) bone marrow; they were assigned to two groups. mice in WT group were injected with 106 Tcd bone marrow cells while mice in mHc-/- group were injected with 106 Tcd bone marrow cells. loss of body weight, gVHd clinical scores and the survival rate of each group were monitored.Histology was used to analyze the severity of gVHd.Result①We found that radiation doses at 1000rad was able to induce autoimmune graft-versus-host disease caused by lack of major histocompatibility complex in a mHc mismatched allo-HcT mouse model.②under 1000rad radiation, mice in mHc-/- group showed higher clinical score and shorter survival time;histopathology showed vast lymphocytes infiltration (P<0.05).③under 800rad radiation, there was no significant difference between those two groups in body weight or overall survival rate;neither group showed gVHd(P>0.05).Conclusion lack of major histocompatibility complex induced autoimmune graft-versus-host disease is related to radiation doses.