CAJ | 학술논문

目的：探讨热毒宁注射液抗菌及提高免疫力作用。方法：将小鼠随机分为正常组、模型组、双黄连组（7.8 mL·kg-1）和热毒宁注射液高、中、低剂量组（20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1），采用体内抗菌实验观察其对细菌感染的小鼠死亡保护作用；将小鼠随机分为正常组、模型组、香菇多糖组（0.19 mg·kg-1）和热毒宁注射液高、中、低剂量组（20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1），采用2，4-二硝基氯苯所致小鼠迟发型超敏反应、小鼠血清溶血素方法及碳粒廓清实验观察其免疫调节作用。结果：热毒宁注射液高、中剂量组具有抗菌作用，可降低小鼠死亡率；热毒宁注射液高、中、低剂量组可增强免疫低下小鼠巨噬细胞的吞噬能力，能增加小鼠溶血素的生成，增强免疫低下小鼠迟发型的超敏反应。结论：热毒宁注射液具有抗菌，增强小鼠非特异性免疫、体液免疫和细胞免疫作用等免疫调节作用。
목적：탐토열독저주사액항균급제고면역력작용。방법：장소서수궤분위정상조、모형조、쌍황련조（7.8 mL·kg-1）화열독저주사액고、중、저제량조（20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1），채용체내항균실험관찰기대세균감염적소서사망보호작용；장소서수궤분위정상조、모형조、향고다당조（0.19 mg·kg-1）화열독저주사액고、중、저제량조（20.30 g·kg-1、10.15 g·kg-1、5.08 g·kg-1），채용2，4-이초기록분소치소서지발형초민반응、소서혈청용혈소방법급탄립곽청실험관찰기면역조절작용。결과：열독저주사액고、중제량조구유항균작용，가강저소서사망솔；열독저주사액고、중、저제량조가증강면역저하소서거서세포적탄서능력，능증가소서용혈소적생성，증강면역저하소서지발형적초민반응。결론：열독저주사액구유항균，증강소서비특이성면역、체액면역화세포면역작용등면역조절작용。
This study was aimed to discuss the antibacterial and immunomodulation effect ofRe-Du-Ning (RDN) injection. Mice were randomly divided into the normal group, model group,Shuang-Huang-Lian (SHL) group (7.8 mL·kg-1), RDN high dose group (20.30 g·kg-1), middle dose group (10.15 g·kg-1), and low dose group (5.08 g·kg-1), in order to observe the death protective effect of mice with bacterial infection on antibacterial experimentin vivo. Mice were randomly divided into the normal group, model group,Xiang-Gu Duo-Tang (XGDT) group (0.19 mg·kg-1), RDN high dose group (20.30 g·kg-1), middle dose group (10.15 g·kg-1), and low dose group (5.08 g·kg-1). The 2, 4-dinitrochlorobenzene was used to induce delayed hypersensitivity. Immunomodulation was observed by the content of serum hemolysin and the carbon particle clearance index. The results showed that the RDN high dose group and middle dose group had antibacterial effect, which reduced the mortality of mice. The RDN high dose, middle dose and low dose group can enhance the phagocytosis of macrophage in immunosuppressive mice, increase the formation of hemolysin, and strengthen delayed hypersensitivity reaction among immunocompromised mice. It was concluded that RDN injection had antibacterial effect. Its immunomodulation effect was through the enhancing of non-specific immunity, humoral immunity and cellular immunity of mice.