世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2015年
5期
1051-1055
,共5页
顾睿%李石平%倪付勇%赵祎武%曹亮%黄文哲%王振中%徐筱杰%萧伟
顧睿%李石平%倪付勇%趙祎武%曹亮%黃文哲%王振中%徐篠傑%蕭偉
고예%리석평%예부용%조의무%조량%황문철%왕진중%서소걸%소위
分子印迹技术%溶胶-凝胶法%芍药苷%桂枝茯苓胶囊
分子印跡技術%溶膠-凝膠法%芍藥苷%桂枝茯苓膠囊
분자인적기술%용효-응효법%작약감%계지복령효낭
Molecular imprinted technology%sol-gel process%paeoniflorin%Gui-Zhi Fu-Ling Capsule
目的：芍药苷类化学成分是桂枝茯苓胶囊中主要活性成分，研究从桂枝茯苓胶囊中定向分离芍药苷及其结构类似物的方法。方法：应用溶胶-凝胶法制备芍药苷分子印迹聚合物，并考察其吸附性能，同时利用该分子印迹聚合物从桂枝茯苓胶囊甲醇溶液中直接分离得到芍药苷活性组分。结果：合成芍药苷分子印迹聚合物（MIP）最大表观结合位点数Qmax=52.28 mg·g-1，4 g桂枝茯苓胶囊甲醇溶液通过一步分离，共制得芍药苷及其结构类似物197 mg，芍药苷纯度89.3%。结论：芍药苷分子印迹聚合物能够从桂枝茯苓胶囊中定向分离得到芍药苷类成分。
目的：芍藥苷類化學成分是桂枝茯苓膠囊中主要活性成分，研究從桂枝茯苓膠囊中定嚮分離芍藥苷及其結構類似物的方法。方法：應用溶膠-凝膠法製備芍藥苷分子印跡聚閤物，併攷察其吸附性能，同時利用該分子印跡聚閤物從桂枝茯苓膠囊甲醇溶液中直接分離得到芍藥苷活性組分。結果：閤成芍藥苷分子印跡聚閤物（MIP）最大錶觀結閤位點數Qmax=52.28 mg·g-1，4 g桂枝茯苓膠囊甲醇溶液通過一步分離，共製得芍藥苷及其結構類似物197 mg，芍藥苷純度89.3%。結論：芍藥苷分子印跡聚閤物能夠從桂枝茯苓膠囊中定嚮分離得到芍藥苷類成分。
목적：작약감류화학성분시계지복령효낭중주요활성성분，연구종계지복령효낭중정향분리작약감급기결구유사물적방법。방법：응용용효-응효법제비작약감분자인적취합물，병고찰기흡부성능，동시이용해분자인적취합물종계지복령효낭갑순용액중직접분리득도작약감활성조분。결과：합성작약감분자인적취합물（MIP）최대표관결합위점수Qmax=52.28 mg·g-1，4 g계지복령효낭갑순용액통과일보분리，공제득작약감급기결구유사물197 mg，작약감순도89.3%。결론：작약감분자인적취합물능구종계지복령효낭중정향분리득도작약감류성분。
Paeoniflorin and its derivatives are main active compounds inGui-Zhi Fu-Ling Capsule (GZFLC). In this study, molecular imprinted polymer (MIP) was prepared by sol-gel process to obtain paeoniflorin and its derivatives in GZFLC. The static adsorption capacity of MIP was measured by scatchard equation. The results showed that the maximum apparent absorbing capacity of MIP was 52.28 mg·g-1. One-step separation of paeoniflorin from 4 g methanol samples of GZFLC was 197 mg with the purity of 89.3%. It was concluded that paeoniflorin MIP can be used to separate phaoniforin and its analogues from GZFLC.
