临床儿科杂志
臨床兒科雜誌
림상인과잡지
2015年
8期
702-705
,共4页
张艳%许秀娟%王伟%邹丽萍
張豔%許秀娟%王偉%鄒麗萍
장염%허수연%왕위%추려평
毛细支气管炎%单核细胞趋化因子%趋化因子受体3%特应性体质
毛細支氣管炎%單覈細胞趨化因子%趨化因子受體3%特應性體質
모세지기관염%단핵세포추화인자%추화인자수체3%특응성체질
bronchiolitis%monokine induced by interferon- γ%chemokine receptor 3%atopy
目的：观察毛细支气管炎患儿血清单核细胞趋化因子(Mig)的水平及外周血淋巴细胞表面趋化因子受体3(CXCR3)的表达。方法选取住院毛细支气管炎患儿55例，按有无特异性体质分为特应性组和非特应性组，以同年龄门诊健康体检儿童26例为对照组。采用流式细胞术测定外周血CD4+、CD8+T淋巴细胞表面CXCR3(CD183)的表达， ELISA法测定血清中CXCR3配体Mig的水平。结果特应性组、非特应性组及对照组的外周血CD4+T细胞表面CD183+的表达分别为(16.39±4.13)%、(14.39±3.74)%和(11.17±3.13)%，差异有统计学意义(P<0.05)；外周血CD8+T细胞表面CD183+的表达分别为(67.18±10.57)%、(61.44±11.46)%和(51.19±5.42)%，差异有统计学意义(P<0.05)；血清Mig水平分别为(99.67±35.77)ng/L、(120.28±32.28)ng/L和(63.90±15.82)ng/L，差异有统计学意义(P<0.05)。结论 Mig和CXCR3均参与了毛细支气管炎的发病过程；CXCR3可能与特应性体质相关。
目的：觀察毛細支氣管炎患兒血清單覈細胞趨化因子(Mig)的水平及外週血淋巴細胞錶麵趨化因子受體3(CXCR3)的錶達。方法選取住院毛細支氣管炎患兒55例，按有無特異性體質分為特應性組和非特應性組，以同年齡門診健康體檢兒童26例為對照組。採用流式細胞術測定外週血CD4+、CD8+T淋巴細胞錶麵CXCR3(CD183)的錶達， ELISA法測定血清中CXCR3配體Mig的水平。結果特應性組、非特應性組及對照組的外週血CD4+T細胞錶麵CD183+的錶達分彆為(16.39±4.13)%、(14.39±3.74)%和(11.17±3.13)%，差異有統計學意義(P<0.05)；外週血CD8+T細胞錶麵CD183+的錶達分彆為(67.18±10.57)%、(61.44±11.46)%和(51.19±5.42)%，差異有統計學意義(P<0.05)；血清Mig水平分彆為(99.67±35.77)ng/L、(120.28±32.28)ng/L和(63.90±15.82)ng/L，差異有統計學意義(P<0.05)。結論 Mig和CXCR3均參與瞭毛細支氣管炎的髮病過程；CXCR3可能與特應性體質相關。
목적：관찰모세지기관염환인혈청단핵세포추화인자(Mig)적수평급외주혈림파세포표면추화인자수체3(CXCR3)적표체。방법선취주원모세지기관염환인55례，안유무특이성체질분위특응성조화비특응성조，이동년령문진건강체검인동26례위대조조。채용류식세포술측정외주혈CD4+、CD8+T림파세포표면CXCR3(CD183)적표체， ELISA법측정혈청중CXCR3배체Mig적수평。결과특응성조、비특응성조급대조조적외주혈CD4+T세포표면CD183+적표체분별위(16.39±4.13)%、(14.39±3.74)%화(11.17±3.13)%，차이유통계학의의(P<0.05)；외주혈CD8+T세포표면CD183+적표체분별위(67.18±10.57)%、(61.44±11.46)%화(51.19±5.42)%，차이유통계학의의(P<0.05)；혈청Mig수평분별위(99.67±35.77)ng/L、(120.28±32.28)ng/L화(63.90±15.82)ng/L，차이유통계학의의(P<0.05)。결론 Mig화CXCR3균삼여료모세지기관염적발병과정；CXCR3가능여특응성체질상관。
ObjectiveTo explore the expression of monokine induced by interferon- γ(Mig) in serum and chemokine receptor 3(CXCR3)on lymphocytes of peripheral blood in children with bronchiolitis.MethodsIn this study, 55 patients with bronchiolitis in our hospital were randomly recuited, and were divided into two groups: atopic group and non-atopic group. Of the same age 26 healthy children had been enrolled randomly as control group. The level of CXCR3 expression (CD183) on lymphocytes of peripheral blood was detected by lfow cytometry in all children. The level of Mig in serum was assayed by ELISA.ResultsThe level of CD183 expression on the CD3+CD4+T lymphocytes in atopic group and non-atopic group(16.39±4.13%,14.39±3.74 %)were higher than that of control group(11.17±3.13%,P<0.05),CD183+CD4+/CD4+% in atopic group were higher than that in non-atopic group(P<0.05). The level of CD183 expression on CD3+CD8+T lymphocytes in atopic group and non-atopic group(67.18±10.57 %, 61.44±11.46 %)were higher than that of control group(51.19±5.42 %, P<0.05),CD183+CD8+/CD8+% in atopic group were signiifcantly higher than that in non-atopic group(P<0.05). The level of Mig in serum of children with bronchiolitis in atopic group and non-atopic group(99.67±35.77ng/L, 120.28±32.28ng/L)were signiifcantly higher than that in control group(63.90±15.82 ng/L,P<0.05). The level of Mig in non-atopic group was higher than that in atopic group, there was signiifcant difference(P<0.05).ConclusionsMig and CXCR3 are involved in the pathogenesis of bronchiolitis, and CXCR3 may relate to allergic factors.
