CAJ | 학술논문

目的：为了更好地控制地菍药材质量，建立地菍中牡荆素和异牡荆素的含量测定方法。方法：高效液相色谱法测定地菍中牡荆素和异牡荆素的含量，色谱柱SunFireTM C18（4.6 mm×150 mm，5μm），检测波长365 nm，流动相为甲醇-0.2%甲酸水溶液，柱温40℃，流速1.0 mL·min-1。结果：牡荆素和异牡荆素的回归曲线分别为：Y=1×106X-14396，Y=1×106X-13900，它们分别在0.210μg-1.050μg（r=0.999）和0.186μg-0.930μg（r=1.000）范围内线性关系良好，加样回收率分别97.48%、104.64%，RSD分别为2.32%、1.51%。样品含量牡荆素和异牡荆素分别为1.25和1.86 mg·g-1.结论：本方法简便可行，重复性好，可用于地菍中牡荆素和异牡荆素的含量测定。
목적：위료경호지공제지녈약재질량，건입지녈중모형소화이모형소적함량측정방법。방법：고효액상색보법측정지녈중모형소화이모형소적함량，색보주SunFireTM C18（4.6 mm×150 mm，5μm），검측파장365 nm，류동상위갑순-0.2%갑산수용액，주온40℃，류속1.0 mL·min-1。결과：모형소화이모형소적회귀곡선분별위：Y=1×106X-14396，Y=1×106X-13900，타문분별재0.210μg-1.050μg（r=0.999）화0.186μg-0.930μg（r=1.000）범위내선성관계량호，가양회수솔분별97.48%、104.64%，RSD분별위2.32%、1.51%。양품함량모형소화이모형소분별위1.25화1.86 mg·g-1.결론：본방법간편가행，중복성호，가용우지녈중모형소화이모형소적함량측정。
This study was aimed to develop HPLC for determination of vitexin and isovitexin inM. dodecandrum. The HPLC column was SunFireTM C18 (4.6 mm× 150 mm, 5μm). The detection wavelength was 365 nm. The mobile phase was methanol-0.2% formic acid aqueous solution. The column temperature was 40℃. The flow rate was 1.0 mL·min-1. The results showed that the regression equations of vitexin and isovitexin wereY = 1× 106X– 14 396, Y = 1× 106X– 13 900, respectively. The linear ranges were 0.210μg - 1.050μg (r = 0.999) and 0.186μg - 0.930μg (r = 1.000), respectively. The recovery rates were 97.48% and 104.64%, respectively. The RSD were 2.32%and 1.51%, respectively. The sample contents of vitexin and isovitexin were 1.25 and 1.86 mg·g1, respectively. It was concluded that the method was simple, feasible and reproducible for the content determination of vitexin and isovitexin inM. dodecandrum.