CAJ | 학술논문

目的：研究不同产地的桔梗药材皂苷指纹图谱，为桔梗的质量控制与生产提供参考依据。方法：采用DB101大孔树脂纯化精制桔梗皂苷，应用HPLC-ELSD法测定全国9省市自治区39批桔梗样品皂苷类化学成分指纹图谱。色谱条件：选用Agilent HC-C8（4.6 mm×250 mm，5μm）柱，流动相为乙腈-0.5%乙酸系统，梯度洗脱；进样量6μL；流速0.5 mL·min-1；漂移管温度90℃；氮气流速1.2 mL·min-1。结果：以内蒙古赤峰产区牛家营子镇6批样品共有指纹图谱为参照，发现各产区桔梗药材品质存在较大差异，其品质与各地桔梗栽培用种种源不规范和生态环境差异及药材干燥、贮藏等初加工方式密切相关。结论：在桔梗栽培生产中，应加强桔梗种源基地建设，规范用种，合理制定桔梗生产区划，规范桔梗产地加工。
목적：연구불동산지적길경약재조감지문도보，위길경적질량공제여생산제공삼고의거。방법：채용DB101대공수지순화정제길경조감，응용HPLC-ELSD법측정전국9성시자치구39비길경양품조감류화학성분지문도보。색보조건：선용Agilent HC-C8（4.6 mm×250 mm，5μm）주，류동상위을정-0.5%을산계통，제도세탈；진양량6μL；류속0.5 mL·min-1；표이관온도90℃；담기류속1.2 mL·min-1。결과：이내몽고적봉산구우가영자진6비양품공유지문도보위삼조，발현각산구길경약재품질존재교대차이，기품질여각지길경재배용충충원불규범화생태배경차이급약재간조、저장등초가공방식밀절상관。결론：재길경재배생산중，응가강길경충원기지건설，규범용충，합리제정길경생산구화，규범길경산지가공。
The HPLC fingerprint differences ofRadix Platycodonis from different origins were studied to provide references for their quality control and production. The total platycodins were purified by DB101 macroporous resin. HPLC-ELSD fingerprints of the total platycodins for 39 batches ofRadix Platycodonis samples from 9 provinces were performed on an Agilent HC-C8 column (4.6 mm x 250 mm, 5μm) with gradient elution. The mobile phase was acetonitrile-0.5% acetic acid. The injection volume was 6μL. The flow rate was 0.5 mL·min-1. The temperature of drift tube was set at 90℃. And the gas flow (N2) was set at 1.2 mL·min-1. The results showed that there were large differences in the quality ofRadix Platycodonis from different origins with the common fingerprints of 6 batches of samples fromChifeng in Inner Mongolia as references. The quality ofRadix Platycodonis was closely related to the seeds, the ecological environment, the way of drying and storing and so on. It was concluded that it was important to strengthen the provenance base construction, standardization of the seeds, reasonable formulation of the regionalization, and standardization of the production processing for the cultivation and production ofRadix Platycodonis.