CAJ | 학술논문

目的：建立丹参胶囊脂溶性成分的高效液相色谱指纹图谱，用来全面评价丹参胶囊脂溶性成分的质量。方法：采用高效液相色谱法，以Welch ultimate XB-C18（250 mm×4.6 mm，5μm）为色谱柱，乙腈-水为流动相进行梯度洗脱。柱温为25℃，流速为1 mL·min-1，检测波长为270 nm，进样量为10μL。结果：分别确立了两个厂家丹参胶囊脂溶性成分指纹图谱中的7个和11个共有峰，20批样品的相似度均在0.9以上。结论：该分析方法稳定性和重现性较好，可作为评价丹参胶囊脂溶性成分质量控制的依据。
목적：건립단삼효낭지용성성분적고효액상색보지문도보，용래전면평개단삼효낭지용성성분적질량。방법：채용고효액상색보법，이Welch ultimate XB-C18（250 mm×4.6 mm，5μm）위색보주，을정-수위류동상진행제도세탈。주온위25℃，류속위1 mL·min-1，검측파장위270 nm，진양량위10μL。결과：분별학립료량개엄가단삼효낭지용성성분지문도보중적7개화11개공유봉，20비양품적상사도균재0.9이상。결론：해분석방법은정성화중현성교호，가작위평개단삼효낭지용성성분질량공제적의거。
This study was aimed to establish the HPLC fingerprint of liposoluble components inDanshen capsule for quality evaluation. HPLC was run on a Welch ultimate XB-C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-water as mobile phase in gradient elution at a flow rate of 1 mL·min-1. The column temperature was 25℃. The detection wavelength was 270 nm. The injection volume was 10μL. The results showed that 7 and 11 common peaks were selected as the HPLC fingerprint of liposoluble components inDanshen capsule from two manufactures, respectively. The similarities of 20 batches of samples were above 0.9. It was concluded that the analysis was stable and reproducible, which can be used as a basis for evaluating the quality control of liposoluble components in Danshen capsule.