CAJ | 학술논문

目的：探讨血管内皮生长因子( VEGF )及其磷酸化受体表达在肝外胆管细胞癌(EHCC)中的临床意义。方法免疫组化法检测VEGF及其磷酸化受体pVEGFR1/2在35例EHCC患者组织中的表达情况,Western blotting检测EHCC细胞系中VEGF及其相关受体的表达情况,探讨VEGF及其受体蛋白的表达与肿瘤进展、预后的相关性。结果 VEGF、pVEGFR1和pVEGFR2阳性的病例数分别为20(57.1%)、14(40.0%)和14(40.0%)例。 pVEGFR1的高表达与细胞增殖相关蛋白pErk1/2水平、神经侵犯、TNM分期、肿瘤分化程度、静脉侵犯程度均相关(P均小于0.05)。pVEGFR1的高表达增加EHCC患者的死亡风险(HR=4.45,95%CI 38.66~61.62,P=0.035)。结论pVEGFR1/2的表达程度与EHCC的进展呈正相关,高表达增加患者死亡风险,提示pVEGFR1/2有可能作为EHCC患者临床预后的预测因子。
목적：탐토혈관내피생장인자( VEGF )급기린산화수체표체재간외담관세포암(EHCC)중적림상의의。방법면역조화법검측VEGF급기린산화수체pVEGFR1/2재35례EHCC환자조직중적표체정황,Western blotting검측EHCC세포계중VEGF급기상관수체적표체정황,탐토VEGF급기수체단백적표체여종류진전、예후적상관성。결과 VEGF、pVEGFR1화pVEGFR2양성적병례수분별위20(57.1%)、14(40.0%)화14(40.0%)례。 pVEGFR1적고표체여세포증식상관단백pErk1/2수평、신경침범、TNM분기、종류분화정도、정맥침범정도균상관(P균소우0.05)。pVEGFR1적고표체증가EHCC환자적사망풍험(HR=4.45,95%CI 38.66~61.62,P=0.035)。결론pVEGFR1/2적표체정도여EHCC적진전정정상관,고표체증가환자사망풍험,제시pVEGFR1/2유가능작위EHCC환자림상예후적예측인자。
Objective To study the clinical significance of vascular endothelial growth factor (VEGF) and its phosphorylated receptors in extrahepatic cholangiocarcinoma (EHCC). Methods Immuno-histochemistry and Western blotting were used to determine the expression of VEGF and phosphorylated VEGF receptors (pVEGFR1/2) in EHCC tissues or EHCC cell lines of thirty-five cases. Clinical data were collected and further analyzed with protein expression of VEGF and pVEGFR1/2. Results There were 20 (57.1%), 14 (40.0%) and 14 (40.0%) positive cases of VEGF, pVEGFR1 and pVEGFR2, respectively. The over-expression of pVEGFR1 was correlated with pErk1/2, perineural invasion, TNM staging, tumor differen-tiation and vascular invasion (P<0.05). Over-expression of pVEGFR1 showed poor overall survival of EHCC patients (HR=4.45, 95%CI:38.66-61.62, P=0.035). Conclusion In EHCC, pVEGFR1 and pVEGFR2 are correlated with tumor progression and poor prognosis, indicating that VEGF and its receptors may serve as predicting factors for prognosis in EHCC.