CAJ | 학술논문

研究了不同温度条件下脲酶抑制剂氢醌( HQ)对东北3种典型土壤(白浆土、棕壤、褐土)脲酶动力学参数的影响。结果表明，土壤类型、培养时间、培养温度及其相互作用均显著影响土壤脲酶动力学参数。与对照相比，加入HQ使土壤脲酶米氏常数(Km)增加，最大反应速率(Vmax)降低，表明HQ对土壤脲酶的作用机理属于混合型抑制。与白浆土相比，棕壤和褐土脲酶动力学参数受HQ的影响程度较大，表明高肥力土壤生物学活性较稳定。随着培养时间延长，土壤脲酶Km降低， Vmax和Vmax/Km增加。随着温度升高，土壤脲酶Km和Vmax增加， Vamx/Km无规律性变化。相关性分析表明，土壤脲酶动力学参数Km、 Vmax和Vmax/Km与pH值、有机质、全氮、碱解氮和质地组成之间存在显著相关关系。
연구료불동온도조건하뇨매억제제경곤( HQ)대동북3충전형토양(백장토、종양、갈토)뇨매동역학삼수적영향。결과표명，토양류형、배양시간、배양온도급기상호작용균현저영향토양뇨매동역학삼수。여대조상비，가입HQ사토양뇨매미씨상수(Km)증가，최대반응속솔(Vmax)강저，표명HQ대토양뇨매적작용궤리속우혼합형억제。여백장토상비，종양화갈토뇨매동역학삼수수HQ적영향정도교대，표명고비력토양생물학활성교은정。수착배양시간연장，토양뇨매Km강저， Vmax화Vmax/Km증가。수착온도승고，토양뇨매Km화Vmax증가， Vamx/Km무규률성변화。상관성분석표명，토양뇨매동역학삼수Km、 Vmax화Vmax/Km여pH치、유궤질、전담、감해담화질지조성지간존재현저상관관계。
The effect of urease inhibitor quinhydrone ( HQ) on kinetic parameters of urease in three typical soils ( i. e. albic soil, brown soil, cinnamon soil) in Northeast China under different temperature conditions. The results showed that, soil type, incubation time, incubation temperature and their interactions significantly influenced soil urease kinetics. Compared with control, Km increase and Vmax decrease in the soils with HQ and it showed HQ was mixed inhibition to soil urease. Compared with albic soil, in brown soil and cinnamon soil the influence on kinetic parameters of soil urease as affected by HQ was greater, which indicated that high fertility soils could have stable biological characteristics. With incubation time increasing, Km decreased, but Vmax and Vmax/Km increased. With incubation temperature increasing, Km and Vmax in-creased, Vmax/Km changed irregularly. Statistical analysis indicated that, Km, Vmax and Vmax/Km of soil urease had significantly correlations with pH, organic matter, total nitrogen, alkali-hydrolyzed nitrogen and texture structure.