CAJ | 학술논문

目的：明确复方水牛角颗粒治疗急性痛风性关节炎的作用机理。方法：一次性大鼠单侧踝关节内注入尿酸钠盐溶液诱导急性痛风性关节炎模型，正常组注射等量生理盐水。造模后4h，分别给予复方水牛角颗粒、秋水仙碱灌胃，正常组和模型组灌胃等量生理盐水。每日1次，共3天，72 h取大鼠血清，酶联免疫吸附测定（ ELISA）法检测TNF－α、IL－8的蛋白表达。结果：与正常组比较，模型组大鼠血清中TNF－α、IL－8蛋白表达显著升高。与模型组相比，复方水牛角颗粒可明显抑制TNF－α、IL－8的蛋白表达。结论：复方水牛角颗粒具有良好的抗大鼠急性痛风性关节炎作用，其部分作用机制在于：抑制TNF－α、IL－8表达以减少组织的炎性损伤。
목적：명학복방수우각과립치료급성통풍성관절염적작용궤리。방법：일차성대서단측과관절내주입뇨산납염용액유도급성통풍성관절염모형，정상조주사등량생리염수。조모후4h，분별급여복방수우각과립、추수선감관위，정상조화모형조관위등량생리염수。매일1차，공3천，72 h취대서혈청，매련면역흡부측정（ ELISA）법검측TNF－α、IL－8적단백표체。결과：여정상조비교，모형조대서혈청중TNF－α、IL－8단백표체현저승고。여모형조상비，복방수우각과립가명현억제TNF－α、IL－8적단백표체。결론：복방수우각과립구유량호적항대서급성통풍성관절염작용，기부분작용궤제재우：억제TNF－α、IL－8표체이감소조직적염성손상。
Objective:To investigate the mechanism of compound buffalo horns granules for inflammatory reaction in rats with acute gouty arthritis.Methods:Models of acute gouty arthritis were induced by the injection of MSU into rats’ ankle joint cavity.The normal group was injected with the same amount of normal sodium.Four hours after injection, buffalo horns group, colchicine group were given corresponding medicines for three days, while physiological saline was used as control.Three days later, all rats were executed to take serum,measuring levels of TNF-a and IL-8 by the method of ELISA.Results:Compared with rats in the normal group: the levels of TNF-α, IL-8 expressions in blood serum of model rats increased obviously(P<0.01).Compared with rats in the model group: the levels of TNF-α, IL-8 ex-pressions in blood serum of rats in the buffalo horns group and colchicine group were decreased significantly.Conclu-sion:Compound buffalo horns granules have a good effect on rats with acute gouty arthritis, and the effective mechanism is to reduce the expressions of TNF-αand IL-8 in blood serum.