CAJ | 학술논문

目的：研究归芪多糖对衰老的WI－38细胞的细胞周期及p53、p16蛋白表达的影响。方法：采用MTT法，研究不同浓度归芪多糖对WI－38细胞活力的影响，筛选出归芪多糖最佳作用浓度；采用流式细胞术分析细胞周期；Western blot法分析归芪多糖对p53、p16蛋白表达的影响。结果：0．016／mg· mL归芪多糖可明显促进细胞增殖；与衰老组细胞相比，归芪多糖可明显促进衰老细胞进入分裂期（ P＜0．01），并可显著降低衰老模型组细胞p53和p16蛋白的表达。结论：归芪多糖可延缓WI－38细胞的衰老，其作用机制与调控细胞周期，抑制p53－p21和p16－pRb信号通路有关。
목적：연구귀기다당대쇠로적WI－38세포적세포주기급p53、p16단백표체적영향。방법：채용MTT법，연구불동농도귀기다당대WI－38세포활력적영향，사선출귀기다당최가작용농도；채용류식세포술분석세포주기；Western blot법분석귀기다당대p53、p16단백표체적영향。결과：0．016／mg· mL귀기다당가명현촉진세포증식；여쇠로조세포상비，귀기다당가명현촉진쇠로세포진입분렬기（ P＜0．01），병가현저강저쇠로모형조세포p53화p16단백적표체。결론：귀기다당가연완WI－38세포적쇠로，기작용궤제여조공세포주기，억제p53－p21화p16－pRb신호통로유관。
Objective:To investigate the effect of Guiqi polysaccharide ( GQP) on the cell cycle and the protein expres-sion of p53 and p16 in senescence cells.Methods:MTT assay was used to observe the effect of different concentrations of GQP on the proliferation of WI-38 cells and screen the best concentration of GQP to promote the growth of WI-38 cells.Cell cycle was analyzed by flow cytometry, and Western blot analysis was performed to evaluate the protein ex-pression of p53 and p16 in senescence cells.Results:0.016mg/mL GQP was the best concentration of promoting cell proliferation.Compared with H2 O2 model group, GQP could promote the senescence cells from G1-phase to S-phase and reduce the protein expression of p53 and p16, and the difference was significant ( P<0.01) .Conclusion:GQP has an protective effect on senescence cells.The anti-aging effect of GQP may be involved in regulating the cell cycle and weakening two signal pathways ( p53-p21 and p16-pRb) .