CAJ | 학술논문

目的：观察防己水煎液及其不同拆分部位对脂多糖（LPS）诱导下RAW264．7细胞炎症因子的影响。方法：采用噻唑蓝比色法（ MTT法）测定防己水煎液及其不同拆分部位对RAW264．7细胞的毒性作用，格里斯氏试剂（ Griess法）测定细胞炎症反应产生的NO含量，酶联免疫吸附法（ ELISA）检测细胞上清液中肿瘤坏死因子－α（TNF－α），白介素－6（IL－6）的含量。结果：防己水煎液及其生物碱拆分组分可抑制LPS诱导的RAW264．7细胞释放细胞炎症因子NO（P＜0．01），TNF－α（P＜0．01），IL－6（P＜0．01），其他组分无明显效果。结论：防己水煎液及其不同拆分部位在浓度小于312．50mg／L的范围内对RAW264．7细胞无显著毒性。防己水煎液及其生物碱拆分组分可能通过抑制细胞因子NO，TNF－α，IL－6的释放发挥其抗炎作用。
목적：관찰방기수전액급기불동탁분부위대지다당（LPS）유도하RAW264．7세포염증인자적영향。방법：채용새서람비색법（ MTT법）측정방기수전액급기불동탁분부위대RAW264．7세포적독성작용，격리사씨시제（ Griess법）측정세포염증반응산생적NO함량，매련면역흡부법（ ELISA）검측세포상청액중종류배사인자－α（TNF－α），백개소－6（IL－6）적함량。결과：방기수전액급기생물감탁분조분가억제LPS유도적RAW264．7세포석방세포염증인자NO（P＜0．01），TNF－α（P＜0．01），IL－6（P＜0．01），기타조분무명현효과。결론：방기수전액급기불동탁분부위재농도소우312．50mg／L적범위내대RAW264．7세포무현저독성。방기수전액급기생물감탁분조분가능통과억제세포인자NO，TNF－α，IL－6적석방발휘기항염작용。
Objective:To observe the effect of inflammatory factors from decoction of Stephania tetrandra S.Moore and its different split components that lipopolysaccharide (LPS)was induced by RAW264.7 cells.Methods:Use MTT method to determine the cytotoxicity of different parts from the decoction and its split components of RAW264.7 cells.Use Gries'reagent ( Griess method) to determine the NO content of the inflammatory reaction cell and use the method of enzyme-linked immunosorbent (ELISA) to detect tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) content in the supernatant fluid.Results: The decoction and its alkaloids components could inhibit LPS induced by RAW264.7 cells releasing inflammatory factor NO ( P<0.01) , the TNF-α( P<0.01) , IL-6 ( P<0.01) and other components had no obvious effects.Conclusion:The concentrations of decoction and its different parts are less than 312.50mg/L for RAW264.7 cells and have no significant toxicity.The decoction and its alkaloids split components may inhibit the re-lease of cytokines NO, TNF-α, IL-6 and exert its anti-inflammatory effects.