世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2015年
5期
963-970
,共8页
倪力军%朱婷婷%王南南%张立国
倪力軍%硃婷婷%王南南%張立國
예력군%주정정%왕남남%장입국
腰痛宁胶囊%有效部位%免疫调节%抗炎%骨关节炎
腰痛寧膠囊%有效部位%免疫調節%抗炎%骨關節炎
요통저효낭%유효부위%면역조절%항염%골관절염
Yao-Tong-Ning Capsule%active fractions%immune regulation%anti-inflammation%osteoarthritis
目的：评价腰痛宁胶囊组方、拆方及其有效部位对细胞增殖以及免疫、抗炎和骨细胞修复功能的影响。方法：按照腰痛宁胶囊组方原则及组合化学思想，根据腰痛宁组方药材比例配制了由腰痛宁不同类型有效部位组成的6个腰痛宁组方及拆方样品，采用半数有效浓度（EC50）或半数抑制浓度（IC50）评价各样品对巨噬（Ana-1）细胞分泌IL-1β、IL-6、TNF-α炎症因子的促进作用；对脂多糖（LPS）诱导的Ana-1细胞释放前列腺素E2（PGE2）产生的抑制作用；对IL-1β诱导的软骨细胞增殖及葡萄糖胺聚糖蛋白（GAG）合成的影响以及对IL-1β诱导的滑膜细胞增殖及IL-6分泌水平的影响，通过比较各样品的EC50（或IC50）叠加值与实验值间的差异分析各有效部位间的相互作用。结果：同一药理模型下6个样品的活性存在差异，部分腰痛宁拆方的某些药理活性优于或显著优于腰痛宁全方，但腰痛宁全方在促进细胞免疫、抗炎及骨细胞修复方面均有良好的功效，腰痛宁药引黄酒有促进各有效部位协同增效的作用。结论：腰痛宁胶囊组方中各有效部位间的协同增效作用是腰痛宁全方具有增强免疫、抗炎及促进骨细胞修复等综合疗效的物质基础。
目的：評價腰痛寧膠囊組方、拆方及其有效部位對細胞增殖以及免疫、抗炎和骨細胞脩複功能的影響。方法：按照腰痛寧膠囊組方原則及組閤化學思想，根據腰痛寧組方藥材比例配製瞭由腰痛寧不同類型有效部位組成的6箇腰痛寧組方及拆方樣品，採用半數有效濃度（EC50）或半數抑製濃度（IC50）評價各樣品對巨噬（Ana-1）細胞分泌IL-1β、IL-6、TNF-α炎癥因子的促進作用；對脂多糖（LPS）誘導的Ana-1細胞釋放前列腺素E2（PGE2）產生的抑製作用；對IL-1β誘導的軟骨細胞增殖及葡萄糖胺聚糖蛋白（GAG）閤成的影響以及對IL-1β誘導的滑膜細胞增殖及IL-6分泌水平的影響，通過比較各樣品的EC50（或IC50）疊加值與實驗值間的差異分析各有效部位間的相互作用。結果：同一藥理模型下6箇樣品的活性存在差異，部分腰痛寧拆方的某些藥理活性優于或顯著優于腰痛寧全方，但腰痛寧全方在促進細胞免疫、抗炎及骨細胞脩複方麵均有良好的功效，腰痛寧藥引黃酒有促進各有效部位協同增效的作用。結論：腰痛寧膠囊組方中各有效部位間的協同增效作用是腰痛寧全方具有增彊免疫、抗炎及促進骨細胞脩複等綜閤療效的物質基礎。
목적：평개요통저효낭조방、탁방급기유효부위대세포증식이급면역、항염화골세포수복공능적영향。방법：안조요통저효낭조방원칙급조합화학사상，근거요통저조방약재비례배제료유요통저불동류형유효부위조성적6개요통저조방급탁방양품，채용반수유효농도（EC50）혹반수억제농도（IC50）평개각양품대거서（Ana-1）세포분비IL-1β、IL-6、TNF-α염증인자적촉진작용；대지다당（LPS）유도적Ana-1세포석방전렬선소E2（PGE2）산생적억제작용；대IL-1β유도적연골세포증식급포도당알취당단백（GAG）합성적영향이급대IL-1β유도적활막세포증식급IL-6분비수평적영향，통과비교각양품적EC50（혹IC50）첩가치여실험치간적차이분석각유효부위간적상호작용。결과：동일약리모형하6개양품적활성존재차이，부분요통저탁방적모사약리활성우우혹현저우우요통저전방，단요통저전방재촉진세포면역、항염급골세포수복방면균유량호적공효，요통저약인황주유촉진각유효부위협동증효적작용。결론：요통저효낭조방중각유효부위간적협동증효작용시요통저전방구유증강면역、항염급촉진골세포수복등종합료효적물질기출。
This study was aimed to evaluate the effects ofYao-Tong-Ning Capsule (YTNC) whole formula, dissembled YTNC formulas, and their active fractions on cell proliferation, immunity, anti-inflammation and osteocytes repair. According to the formulating principle of YTNC and combinatorial chemistry concepts, six samples were prepared by combining different active fractions. Half-maximal effective concentrations (EC50) or half-maximal inhibitory concentrations (IC50) were applied to evaluate the effects of samples on promoting the secretion of IL-1β, IL-6 and TNF-α in macrophage (Ana-1) cells, on inhibiting the production of lipopolysaccharide (LPS)-induced PGE2 in Ana-1 cells, on promoting the cell proliferation induced by IL-1β and glycosaminoglycan (GAG) synthesis, on influencing synoviocytes proliferation induced by IL-1β, and on promoting the secretion of IL-6, respectively. The interactions among the active fractions in these samples were investigated by comparing the additive EC50 (or IC50) values with their experimental EC50 (or IC50). The results showed that pharmacological activities of the six samples were different in the same cell model. Some pharmacological activities of a few dissembled YTNC formulas were superior or significantly superior to the YTNC whole formula. However, the YTNC whole formula was good at promoting cell immunity, anti-inflammation and osteocytes repair. The YTNC’s vehicle Chinese rice wine played an important role on strengthening the activity of YTNC. It was concluded that the synergistic effects between active fractions in YTNC were the material foundation that YTNC had comprehensive efficacy of strengthening immunity, anti-inflammation and promoting osteocytes repair.
