CAJ | 학술논문

利用组织切片及超薄切片电镜技术对患血卵涡鞭虫病拟穴青蟹的肝胰腺、步足肌、心肌和鳃4种组织进行病理学分析，探讨该病的致病机理。组织病理显示：呈变质性炎症，肝小管缩小，空泡化；心肌、步足肌肌纤维断裂，排列疏松；鳃上皮组织变性、坏死；在肝小管管腔间、肌纤维间和鳃腔中寄生有大量血卵涡鞭虫。超微病理显示：肝胰腺细胞形态不规则，细胞核萎缩；心肌、步足肌肌丝断裂，线粒体脊断裂、萎缩、空泡化；鳃组织细胞结构破坏，线粒体数量减少。实验结果表明：拟穴青蟹血卵涡鞭虫病的组织细胞结构发生明显的病理变化，伴随有毒代谢产物的释放，病理损伤加重，病蟹的摄食能力逐渐衰退或丧失，终致死亡。
이용조직절편급초박절편전경기술대환혈란와편충병의혈청해적간이선、보족기、심기화새4충조직진행병이학분석，탐토해병적치병궤리。조직병리현시：정변질성염증，간소관축소，공포화；심기、보족기기섬유단렬，배렬소송；새상피조직변성、배사；재간소관관강간、기섬유간화새강중기생유대량혈란와편충。초미병리현시：간이선세포형태불규칙，세포핵위축；심기、보족기기사단렬，선립체척단렬、위축、공포화；새조직세포결구파배，선립체수량감소。실험결과표명：의혈청해혈란와편충병적조직세포결구발생명현적병리변화，반수유독대사산물적석방，병리손상가중，병해적섭식능력축점쇠퇴혹상실，종치사망。
Tissues from the hepatopancreas, paraeiopod, heart and gill of Scylla paramamosain with Hematodinium-infection were examined histologically to elucidate the pathogenic role of this parasite. Conventional histological examination with light microsco-py revealed that the infected crab had liver inflammation with the shrunk hepatic ducts and necrotic hepatic cells;the cardiac and skel-eton muscle cells were destructed;and gill epithelial cells showed signs of necrosis. The organism, Hematodiniumsp., was seen in tissues. Transmission electron microscopic study confirmed the infection of Hematodinium sp. The ultrastructural examination showed pyknosis in hepatic cells, the destructions of muscular fibers, injury of mitochondria, decreasing number of mitochondria and necrosis of cells of gill tissue. The examinations confirmed that the profound alterations occurred in the crab in the wake of the infec-tion of Hematodiniumsp.