CAJ | 학술논문

采用双波长替代单波长,对考马斯亮蓝分光光度法测定蛋白质技术进行改进。结果表明：以酪蛋白为标准蛋白,选取600 nm和460 nm为测定波长和参比波长,建立了双波法标准曲线,工作曲线相关系数的平方R2=0.999,检测范围3~250μg/mL；干扰试验和重复实验说明该方法准确性高、稳定性好；利用双波长法测定牛奶样品中蛋白质含量,相对偏差小于2.720%。因此,双波长分光光度法是一种简便、快速、准确、实用的蛋白质含量测定方法。
채용쌍파장체대단파장,대고마사량람분광광도법측정단백질기술진행개진。결과표명：이락단백위표준단백,선취600 nm화460 nm위측정파장화삼비파장,건립료쌍파법표준곡선,공작곡선상관계수적평방R2=0.999,검측범위3~250μg/mL；간우시험화중복실험설명해방법준학성고、은정성호；이용쌍파장법측정우내양품중단백질함량,상대편차소우2.720%。인차,쌍파장분광광도법시일충간편、쾌속、준학、실용적단백질함량측정방법。
To improve a method for determination protein content, dual-wavelength spectrophotometry was used instead of single wave?length spectrophotometry in this paper. The results showed that standard curve of double wavelength spectrophotometry was established for the standard protein casein when chosen wavelength of 600 nm and 460 nm. The standard curve was significant for the square of cor?relation coefficient R2=0.999 when protein content in the range of 3-250 μg/mL. It showed that this method was of high accuracy through interference test and repeat experiment. Applying dual wavelength method to assay protein content for fresh milk , the relative standard deviation was less than 2.72%. Therefore, the dual-wavelength spectrophotometry is a good and practical determination meth?od for protein content.