CAJ | 학술논문

血凝素（Hemagglutinin, HA）蛋白是禽流感病毒（Avian Influenza Virus, AIV)最主要的保护性抗原,它不仅可以诱导产生特异性的抗体,还可以刺激机体产生细胞毒性T淋巴细胞（Cytotoxic lymphocyte, CTL）反应。探索禽流感ha在植物中的表达,提高禽流感ha的表达量,对于研制禽流感亚单位疫苗具有重要意义。主要进行了ha的密码子偏好优化,载体构建和烟草中瞬时表达,最终在烟草叶片RNA水平上检测到ha的转录,并通过Western Blot方法检测到目的蛋白的表达。此外,添加Kozak序列和删除载体上的除草剂抗性基因（Bialaphos resistance gene, bar）能提高ha的翻译水平。因此,烟草作为植物生物反应器表达HA具有重要的应用前景。
혈응소（Hemagglutinin, HA）단백시금류감병독（Avian Influenza Virus, AIV)최주요적보호성항원,타불부가이유도산생특이성적항체,환가이자격궤체산생세포독성T림파세포（Cytotoxic lymphocyte, CTL）반응。탐색금류감ha재식물중적표체,제고금류감ha적표체량,대우연제금류감아단위역묘구유중요의의。주요진행료ha적밀마자편호우화,재체구건화연초중순시표체,최종재연초협편RNA수평상검측도ha적전록,병통과Western Blot방법검측도목적단백적표체。차외,첨가Kozak서렬화산제재체상적제초제항성기인（Bialaphos resistance gene, bar）능제고ha적번역수평。인차,연초작위식물생물반응기표체HA구유중요적응용전경。
Hemagglutinin (HA) is the main protective antigen of avian influenza virus. It can not only induce the production of specif-ic antibodies, but also stimulate the response of cytotoxic lymphocyte (CTL) in the body. It is important for the manufacture of avain influenza subunit vaccines to explore the expression of ha in plants and improve the expression quantity of ha. This study focused on the optimization of the codons, the construction of vectors and the transient expression in Nicotianabenthamiana. Lastly, the expres-sion of the ha on the transcriptional level was detected by RT-PCR and the translational level by Western blotting assay. Besides this, the addition of Kozak sequence and the deletion of the bialaphos resistance gene (bar) could improve the translation level of the ha. So, Nicotianabenthamianahas an important application prospect to express the HA as a plant bioreactor.