CAJ | 학술논문

目的：建立并评价肥胖型多囊卵巢综合征（PCOS）大鼠模型。方法：将大鼠随机分为空白对照组、高脂模型组、胰岛素（INS）联合人绒毛膜促性腺激素（HCG）模型组和INS联合HCG加高脂乳剂模型组。通过颈背部分别皮下注射INS和HCG加高脂乳剂灌胃导形成肥胖PCOS大鼠模型。监测大鼠动情周期，测定体质量增加幅度、血清黄体生成素（LH）、卵泡刺激素（FSH）、雌二醇（E2）、睾酮（T）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、甘油三酯（TG）、胆固醇（TC）、空腹血糖（FPG）、空腹胰岛素（FINS）、2 h血糖、2 h INS，计算稳态模型（HOMA）指数、相关脏器指数水平和双侧卵巢HE染色。结果：INS联合HCG改良法（INS联合HCG加高脂模型组）大鼠取材时，体质量、体质量增加幅度、卵巢脏器指数、T、LH、HOMA指数均显著高于空白对照组、高脂模型组和INS联合HCG模型组（P<0.05，P<0.01），HDL明显降低（P<0.05，P<0.01）。INS联合HCG改良法大鼠FPG、FINS、2 h INS均极显著高于高脂模型组（P<0.01）。结论：INS联合HCG改良法诱导的动物模型是研究肥胖型PCOS发病机制的较理想动物模型之一。
목적：건립병평개비반형다낭란소종합정（PCOS）대서모형。방법：장대서수궤분위공백대조조、고지모형조、이도소（INS）연합인융모막촉성선격소（HCG）모형조화INS연합HCG가고지유제모형조。통과경배부분별피하주사INS화HCG가고지유제관위도형성비반PCOS대서모형。감측대서동정주기，측정체질량증가폭도、혈청황체생성소（LH）、란포자격소（FSH）、자이순（E2）、고동（T）、고밀도지단백（HDL）、저밀도지단백（LDL）、감유삼지（TG）、담고순（TC）、공복혈당（FPG）、공복이도소（FINS）、2 h혈당、2 h INS，계산은태모형（HOMA）지수、상관장기지수수평화쌍측란소HE염색。결과：INS연합HCG개량법（INS연합HCG가고지모형조）대서취재시，체질량、체질량증가폭도、란소장기지수、T、LH、HOMA지수균현저고우공백대조조、고지모형조화INS연합HCG모형조（P<0.05，P<0.01），HDL명현강저（P<0.05，P<0.01）。INS연합HCG개량법대서FPG、FINS、2 h INS균겁현저고우고지모형조（P<0.01）。결론：INS연합HCG개량법유도적동물모형시연구비반형PCOS발병궤제적교이상동물모형지일。
This study was aimed to establish and evaluate obese polycystic ovary syndrome (PCOS) rat model. Rats were randomly divided into the blank control group, high-fat model group, insulin (INS) combined human chorionic gonadotropin (HCG) model group, and INS combined HCG plus high fat emulsion model group. The obese PCOS rat model was induced by subcutaneous injection of INS and HCG on the nape, respectively. The intragastric administration of high fat emulsion was also used in the PCOS rat model establishment. The estrous cycle of rat was monitored. The detection was also made the weight increasing rate of rats, luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), testosterone (T), high-density lipoprotein (HDL), low density lipoprotein (LDL), triglyceride (TG), cholesterol (TC), fasting plasma glucose (FPG), fasting insulin (FINS), 2 h blood glucose and 2 h INS. Calculation was made on the HOMA index, related viscera index and bilateral ovaries HE staining. The results showed that when INS combined HCG improved method (INS combined HCG plus high fat emulsion model group) rats were sacrificed, the body weight, weight increasing rate, ovary viscera index, T, LH, and HOMA index were significantly higher than that of the blank control group, high-fat model group and INS combined HCG model group (P < 0.05,P < 0.01); HDL was significantly decreased (P < 0.05,P < 0.01). FPG, FINS, and 2 h INS of the INS combined HCG improved method group were significantly higher than that of the high-fat model group (P <0.01). It was concluded that INS combined HCG improved method was one of the ideal animal model establishment methods in the pathogenesis study of PCOS.