CAJ | 학술논문

目的：观察黄芩苷对炎症性肠病小鼠模型Th22细胞比例及IL-22浓度的影响，通过体内和体外实验探讨黄芩苷治疗该病的免疫学机制。方法： C57BL/6小鼠用3.5%葡聚糖硫酸钠（dextran sodium sulfate）建立结肠炎小鼠模型，随机分成空白对照组、模型组、黄芩苷组。应用流式细胞术和酶联免疫吸附试验（ELISA）分别检测各组小鼠Th22细胞比例和外周血血清IL-22的表达；分离小鼠脾脏淋巴细胞，用含黄芩苷（0、10、20、40μmol·L-1）培养基培养48 h,应用流式细胞术检测各组小鼠淋巴细胞中Th22细胞比例。结果：黄芩苷在体内外实验中均降低Th22细胞比例与IL-22的表达。结论：黄芩苷能够在体外和DSS诱导的结肠炎小鼠体内抑制Th22的分化及IL-22的表达，提示黄芩苷对Th22介导的炎症性疾病具有较好的治疗潜力。
목적：관찰황금감대염증성장병소서모형Th22세포비례급IL-22농도적영향，통과체내화체외실험탐토황금감치료해병적면역학궤제。방법： C57BL/6소서용3.5%포취당류산납（dextran sodium sulfate）건립결장염소서모형，수궤분성공백대조조、모형조、황금감조。응용류식세포술화매련면역흡부시험（ELISA）분별검측각조소서Th22세포비례화외주혈혈청IL-22적표체；분리소서비장림파세포，용함황금감（0、10、20、40μmol·L-1）배양기배양48 h,응용류식세포술검측각조소서림파세포중Th22세포비례。결과：황금감재체내외실험중균강저Th22세포비례여IL-22적표체。결론：황금감능구재체외화DSS유도적결장염소서체내억제Th22적분화급IL-22적표체，제시황금감대Th22개도적염증성질병구유교호적치료잠력。
This study was aimed to investigate the effect of baicalin on the proportion of Th22 cells and the concentration of IL-22 bothin vivo andin vitro, in order to explore the immune mechanism of baicalin on inflammatory bowel disease mice model. The 3.5% dextran sodium sulfate (DSS) was used on C57BL/6 mice for the establishment of colitis mice model. Mice were randomly divided into the blank control group, model group, and baicalin group. Flow cytometry and ELISA were used in the detection of the proportion of Th22 cells and concentration of IL-22 in peripheral blood serum, respectively. The spleen lymphocytes of mice were isolated and cultured by baicalin medium (0, 10, 20, 40μM) for 48 h. Flow cytometry was used in the detection of the proportion of Th22 cells. The results showed that baicalin reduced the proportion of Th22 cells and the expression of IL-22 bothin vivo andin vitro experiments. It was concluded that baicalin can inhibit Th22 cell differentiation and expression of IL-22in vitro and DSS-induced colitis mice. It indicated that baicalin had a good treatment potential in Th22 cell-mediated inflammatory diseases.