CAJ | 학술논문

目的：观察滋补脾阴方药对脾阴虚痴呆大鼠脑内N-甲基-D-天冬氨酸受体（NMDAR）亚单位NR1、NR2A、NR2B mRNA表达的影响。方法：运用逆转录聚合酶链反应（RT-PCR）技术，对NMDAR亚单位NR1、NR2A、NR2B mRNA在各脑区的表达状况作相应的检测。结果：痴呆组以及脾阴虚痴呆组大鼠NMDAR亚单位NR1、NR2A、NR2B mRNA在各脑区表达均明显减弱下调（P<0.05）；ZBPYR治疗组NMDAR亚单位NR1、NR2A、NR2B mRNA均明显上调（P<0.05）。结论：使用ZBPYR治疗的大鼠NMDAR mRNA的表达水平在各脑区均明显上调，这表明ZBPYR以提升NMDAR mRNA的方式，使得NMDAR蛋白的含量显著增多，而发挥其抗痴呆作用。
목적：관찰자보비음방약대비음허치태대서뇌내N-갑기-D-천동안산수체（NMDAR）아단위NR1、NR2A、NR2B mRNA표체적영향。방법：운용역전록취합매련반응（RT-PCR）기술，대NMDAR아단위NR1、NR2A、NR2B mRNA재각뇌구적표체상황작상응적검측。결과：치태조이급비음허치태조대서NMDAR아단위NR1、NR2A、NR2B mRNA재각뇌구표체균명현감약하조（P<0.05）；ZBPYR치료조NMDAR아단위NR1、NR2A、NR2B mRNA균명현상조（P<0.05）。결론：사용ZBPYR치료적대서NMDAR mRNA적표체수평재각뇌구균명현상조，저표명ZBPYR이제승NMDAR mRNA적방식，사득NMDAR단백적함량현저증다，이발휘기항치태작용。
This study was aimed to observe the effect ofZi-Bu Pi-Yin Recipe (ZBPYR) on the mRNA expressions of NMDA receptor subunits NR1, NR2A, NR2B in different brain regions of spleen-yin deficiency Alzheimer's Disease (AD) model rats. The levels of NR1, NR2A, NR2B mRNA expressions were detected by using RT-PCR method. The results showed that the levels of NR1, NR2A, NR2B mRNA expressions of AD group and spleen-yin deficiency AD group decreased significantly (P < 0.05). The levels of NR1, NR2A, NR2B mRNA expressions of ZBPYR treatment group increased significantly (P < 0.05). It was concluded that the expression levels of NMDAR mRNA in different brain regions of the ZBPYR treatment group increased significantly, which indicated that ZBPYR may up-regulate the protein expressions of NMDAR by increasing the expression levels of NMDAR mRNA, thereby to play the anti-dementia effect.