中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2015年
4期
376-379
,共4页
乳香%胰腺癌%凋亡%裸鼠
乳香%胰腺癌%凋亡%裸鼠
유향%이선암%조망%라서
Frankincense%pancreatic cancer%apoptosis%nude mice
目的：：研究乳香提取物体内抑制胰腺癌的可能性及其机制。方法：利用人胰腺癌细胞株MIA PaCa-2体外扩增后种植于裸鼠皮下成瘤，将乳香提取物经裸鼠皮下注射，同时设PBS组为对照，观察肿瘤体积变化（其中乳香提取物治疗组6只，对照组5只），12 d后将肿瘤切除后测量肿瘤体积，固定瘤体后切片，通过标记磷酸化组蛋白H3（PHH3）,原位末端转移酶标记技术（TUNEL）行免疫组化分析。结果：治疗组肿瘤体积为（76.67±24.11）mm3，对照组肿瘤体积为（204.80±101.19）mm3；治疗组PHH3阳性细胞数为197.5±40.4，对照组PHH3阳性细胞数为252.8±30.6；治疗组TUNEL阳性细胞数为316.7±117.3，对照组为TUNEL阳性细胞数为100.6±47.2；治疗组与对照组比较，P＜0.05。结论：乳香提取物在体内可以抑制裸鼠胰腺癌的生长，促进胰腺癌细胞的凋亡。
目的：：研究乳香提取物體內抑製胰腺癌的可能性及其機製。方法：利用人胰腺癌細胞株MIA PaCa-2體外擴增後種植于裸鼠皮下成瘤，將乳香提取物經裸鼠皮下註射，同時設PBS組為對照，觀察腫瘤體積變化（其中乳香提取物治療組6隻，對照組5隻），12 d後將腫瘤切除後測量腫瘤體積，固定瘤體後切片，通過標記燐痠化組蛋白H3（PHH3）,原位末耑轉移酶標記技術（TUNEL）行免疫組化分析。結果：治療組腫瘤體積為（76.67±24.11）mm3，對照組腫瘤體積為（204.80±101.19）mm3；治療組PHH3暘性細胞數為197.5±40.4，對照組PHH3暘性細胞數為252.8±30.6；治療組TUNEL暘性細胞數為316.7±117.3，對照組為TUNEL暘性細胞數為100.6±47.2；治療組與對照組比較，P＜0.05。結論：乳香提取物在體內可以抑製裸鼠胰腺癌的生長，促進胰腺癌細胞的凋亡。
목적：：연구유향제취물체내억제이선암적가능성급기궤제。방법：이용인이선암세포주MIA PaCa-2체외확증후충식우라서피하성류，장유향제취물경라서피하주사，동시설PBS조위대조，관찰종류체적변화（기중유향제취물치료조6지，대조조5지），12 d후장종류절제후측량종류체적，고정류체후절편，통과표기린산화조단백H3（PHH3）,원위말단전이매표기기술（TUNEL）행면역조화분석。결과：치료조종류체적위（76.67±24.11）mm3，대조조종류체적위（204.80±101.19）mm3；치료조PHH3양성세포수위197.5±40.4，대조조PHH3양성세포수위252.8±30.6；치료조TUNEL양성세포수위316.7±117.3，대조조위TUNEL양성세포수위100.6±47.2；치료조여대조조비교，P＜0.05。결론：유향제취물재체내가이억제라서이선암적생장，촉진이선암세포적조망。
Objective To investigate the inhibitory effect and mechanism of pancreatic cancer in nude mice model with Frankincense extract. Methods MIA PaCa-2 of pancreatic cancer cell lines were implanted into nude mice subcutaneously .The nude mice were randomly assigned to receive either PBS(control) or Frankin?cense extract injection subcutaneously (the number of treatment group was 6, while the control group was 5). Af?ter 12 days, tumors were removed . Tumor volume were mersured. Tumors were fixed and immunohistochemical analysis for PHH3 and TUNEL. Results The tumor size of the treatment group was (76.67 ± 24.11) mm3 while the control group was(204.80±101.19)mm3. The difference is statistically significant (p < 0.05). The number of PHH3-immunoreactive cells of treatment group was (197.5±40.4), while the control group was (252.8±30.6). The difference is statistically significant (P<0.05). The number of TUNEL positive cells in treatment group was (316.7 ± 117.3), while the control group was (100.6 ± 47.2). The difference is statistically significant (P<0.05). Conclusion The Frankincense extract can inhibit the growth of pancreatic cancer in vivo of xenograft murine.
