CAJ | 학술논문

目的：观察丹蒌片对动脉粥样硬化兔血脂、炎症因子及PI3K/AKT信号通路的影响，并探讨丹蒌片抗动脉粥样硬化的机制。方法：雄性日本大耳白兔24只，随机分为正常对照组、模型组、丹蒌片组，每组8只。正常对照组饲喂普通饲料，其余各组高脂饲料喂养制备动脉粥样硬化模型；除高脂饲料喂养外，丹蒌片组（0.5 g·kg-1·d-1）胃饲相应药物。连续给药9周后处死，测各组血脂、血清肿瘤坏死因子（TNF-α）和白介素-6（IL-6）的含量；HE染色观察主动脉病理学改变；Western blot检测主动脉中PI3K和p-AKT的蛋白表达水平。结果：与正常对照组比较，模型组中甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白（LDL）及IL-6、TNF-α的浓度均显著升高（P<0.01），主动脉组织中PI3K、p-AKT的蛋白表达水平明显降低（P<0.01）。与模型组相比，丹蒌片组血脂及IL-6、TNF-α水平明显降低（P<0.05或P<0.01），PI3K、p-AKT的蛋白表达显著升高（P<0.01）。结论：丹蒌片具有降低兔血脂、炎症因子，抗动脉粥样硬化的作用，其作用机制可能与激活PI3K/AKT信号通路相关。
목적：관찰단루편대동맥죽양경화토혈지、염증인자급PI3K/AKT신호통로적영향，병탐토단루편항동맥죽양경화적궤제。방법：웅성일본대이백토24지，수궤분위정상대조조、모형조、단루편조，매조8지。정상대조조사위보통사료，기여각조고지사료위양제비동맥죽양경화모형；제고지사료위양외，단루편조（0.5 g·kg-1·d-1）위사상응약물。련속급약9주후처사，측각조혈지、혈청종류배사인자（TNF-α）화백개소-6（IL-6）적함량；HE염색관찰주동맥병이학개변；Western blot검측주동맥중PI3K화p-AKT적단백표체수평。결과：여정상대조조비교，모형조중감유삼지（TG）、총담고순（TC）화저밀도지단백（LDL）급IL-6、TNF-α적농도균현저승고（P<0.01），주동맥조직중PI3K、p-AKT적단백표체수평명현강저（P<0.01）。여모형조상비，단루편조혈지급IL-6、TNF-α수평명현강저（P<0.05혹P<0.01），PI3K、p-AKT적단백표체현저승고（P<0.01）。결론：단루편구유강저토혈지、염증인자，항동맥죽양경화적작용，기작용궤제가능여격활PI3K/AKT신호통로상관。
This study was aimed to investigate the effect ofDanlou pills on prevent atherosclerosis from hypercholesterolemia rabbit and its relationship with inflammatory factors as well as PI3K/AKT signal pathways. A total of 24 Japanese male white rabbits were randomly divided into the control group (CL), model group (M) and Danlou group (DL), with 8 in each group. Normal diet was given to CL rabbits. High-fat diet was given to rabbits in other groups to establish the atherosclerosis model. Danlou pills (0.5 g·kg-1·d-1) were also given to DL rabbits. Rabbits were sacrificed after 9-week medication. The contents of blood lipid, TNF-α and IL-6 were detected. HE staining was used in the observation of histological changes in the aorta. Western blot was used to observe PI3K and p-AKT expression in the aorta. The results showed that compared with CL, the contents of TG, TC, LDL, IL-6 and TNF-α were significantly increased in M (P < 0.01); PI3K and p-AKT expression in the aorta were significantly decreased (P < 0.01). Compared with M, blood lipid, IL-6 and TNF-α were significantly reduced in DL (P < 0.05, orP < 0.01); PI3K and p-AKT expression were significantly increased (P < 0.01). It was concluded thatDanlou pills had prevention effects on atherosclerosis through reducing blood lipid and inflammatory factors. The action mechanism maybe related to the activation of PI3K/AKT signal pathways.