CAJ | 학술논문

目的：建立同时测定蒺藜中3种成分含量的方法，比较不同生长期、不同产地及不同药用部位的蒺藜中3种指标成分含量，探究采收时间及种植地区的科学性。方法：以蒺藜中3种有效成分，即槲皮素-3-O-β-吡喃葡萄糖基-(1→6)-吡喃葡萄糖苷、异鼠李素-3-O-β-D-吡喃葡萄糖基-(1→6)-吡喃葡萄糖苷、Terrestrinone A2为指标成分，高效液相色谱（HPLC）法测定含量。结果：3种指标成分含量均逐渐递增至6月并达到最大，随后逐渐降低；均表现为茎叶>果>根，根中含量低于检测限；产于内蒙古的蒺藜指标成分含量远远高于其他地区，其次为山东、河北、陕西。结论：该方法简便、准确，具有较好的重复性和稳定性，可为蒺藜质量控制提供参考。
목적：건립동시측정질려중3충성분함량적방법，비교불동생장기、불동산지급불동약용부위적질려중3충지표성분함량，탐구채수시간급충식지구적과학성。방법：이질려중3충유효성분，즉곡피소-3-O-β-필남포도당기-(1→6)-필남포도당감、이서리소-3-O-β-D-필남포도당기-(1→6)-필남포도당감、Terrestrinone A2위지표성분，고효액상색보（HPLC）법측정함량。결과：3충지표성분함량균축점체증지6월병체도최대，수후축점강저；균표현위경협>과>근，근중함량저우검측한；산우내몽고적질려지표성분함량원원고우기타지구，기차위산동、하북、협서。결론：해방법간편、준학，구유교호적중복성화은정성，가위질려질량공제제공삼고。
This study was aimed to establish a method for the content determination of three components in Tribulus terrestris L., in order to compare index components of different growing periods, production places and medical parts for the scientific explanation of the harvesting time and growing places. Contents of three effective components inTribulus terrestris L., which were quercetin-3-O-β-D-gentiobioside, isorhamnetin-3-O-β-D-gentiobioside and Terrestrinone A2, were determined by HPLC. The results showed that contents of three evaluation indexes all gradually increased until June and then started reducing. The contents in all evaluated indexes were in the order of the stem and leaf > fruit > root. And the content in the root was below the detection limit. The content indexes ofTribulus terrestris L. of the Inner Mongolia were much higher than that of other regions. It was followed byShandong,Hebei andShanxi province. It was concluded that the method was simple and accurate with good repeatability and stability, which was suitable for quality control ofTribulus terrestris L.