CAJ | 학술논문

目的：观察不同剂量益气固本胶囊对哮喘小鼠病理及气道重塑中TGF‐β1／Smad信号通路的影响。方法将60只雄性SPF级BALB／c小鼠随机分为正常组、模型组、地塞米松干预组、童康片组、益气固本胶囊高剂量组和益气固本胶囊低剂量组，每组10只。除正常组外，其余各组均以卵清白蛋白致敏和激发复制哮喘模型，于第21天起，在每次激发前1 h分别给予模型组和用药各组以纯净水及药物灌胃，正常组不给予任何处置。应用HE染色观察各组小鼠气道及肺组织的病理学改变，免疫组织化学法检测肺组织TGF‐β1和P‐Smad3蛋白的表达。结果小鼠气道病理切片观察模型组的气管、肺组织病理改变程度高于正常组、地塞米松干预组、童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组，差异有统计学意义（P＜0．05）；地塞米松干预组与益气固本胶囊高剂量组、益气固本胶囊低剂量组相比，差异无统计学意义（P＞0．05）；与正常组相比，TGF‐β1蛋白在模型组、地塞米松干预组、童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组表达水平增强，差异有统计学意义（P＜0．01）；与模型组相比，地塞米松干预组、童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组TGF‐β1蛋白表达水平降低，差异有统计学意义（P＜0．01）；地塞米松干预组与益气固本胶囊高剂量组、益气固本胶囊低剂量组相比，差异均无统计学意义（P＞0．05）；益气固本胶囊高剂量组与益气固本胶囊低剂量组相比，差异无统计学意义（P＞0．05）；与正常组相比，P‐Smad3蛋白在模型组、地塞米松干预组、童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组表达水平增强，差异有统计学意义（P＜0．01）；与模型组相比，地塞米松干预组、童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组P‐Smad3蛋白表达降低，差异有统计学意义（P＜0．01）；地塞米松干预组 P‐Smad3蛋白表达比童康片组、益气固本胶囊高剂量组、益气固本胶囊低剂量组更低，差异有统计学意义（P＜0．01）；童康片组与益气固本胶囊高剂量组相比，差异无统计学意义（P＞0．05）；益气固本胶囊高剂量组与益气固本胶囊低剂量组相比，差异无统计学意义（P＞0．05）。结论益气固本胶囊可抑制哮喘小鼠体内TGF‐β1和P‐Smad3的过度表达，从而阻断TGF‐β1胞内信号转导，可在一定程度上减轻哮喘小鼠气道炎症和抑制气道重塑的发生发展。目的：觀察不同劑量益氣固本膠囊對哮喘小鼠病理及氣道重塑中TGF‐β1／Smad信號通路的影響。方法將60隻雄性SPF級BALB／c小鼠隨機分為正常組、模型組、地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組和益氣固本膠囊低劑量組，每組10隻。除正常組外，其餘各組均以卵清白蛋白緻敏和激髮複製哮喘模型，于第21天起，在每次激髮前1 h分彆給予模型組和用藥各組以純淨水及藥物灌胃，正常組不給予任何處置。應用HE染色觀察各組小鼠氣道及肺組織的病理學改變，免疫組織化學法檢測肺組織TGF‐β1和P‐Smad3蛋白的錶達。結果小鼠氣道病理切片觀察模型組的氣管、肺組織病理改變程度高于正常組、地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組，差異有統計學意義（P＜0．05）；地塞米鬆榦預組與益氣固本膠囊高劑量組、益氣固本膠囊低劑量組相比，差異無統計學意義（P＞0．05）；與正常組相比，TGF‐β1蛋白在模型組、地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組錶達水平增彊，差異有統計學意義（P＜0．01）；與模型組相比，地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組TGF‐β1蛋白錶達水平降低，差異有統計學意義（P＜0．01）；地塞米鬆榦預組與益氣固本膠囊高劑量組、益氣固本膠囊低劑量組相比，差異均無統計學意義（P＞0．05）；益氣固本膠囊高劑量組與益氣固本膠囊低劑量組相比，差異無統計學意義（P＞0．05）；與正常組相比，P‐Smad3蛋白在模型組、地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組錶達水平增彊，差異有統計學意義（P＜0．01）；與模型組相比，地塞米鬆榦預組、童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組P‐Smad3蛋白錶達降低，差異有統計學意義（P＜0．01）；地塞米鬆榦預組 P‐Smad3蛋白錶達比童康片組、益氣固本膠囊高劑量組、益氣固本膠囊低劑量組更低，差異有統計學意義（P＜0．01）；童康片組與益氣固本膠囊高劑量組相比，差異無統計學意義（P＞0．05）；益氣固本膠囊高劑量組與益氣固本膠囊低劑量組相比，差異無統計學意義（P＞0．05）。結論益氣固本膠囊可抑製哮喘小鼠體內TGF‐β1和P‐Smad3的過度錶達，從而阻斷TGF‐β1胞內信號轉導，可在一定程度上減輕哮喘小鼠氣道炎癥和抑製氣道重塑的髮生髮展。
목적：관찰불동제량익기고본효낭대효천소서병리급기도중소중TGF‐β1／Smad신호통로적영향。방법장60지웅성SPF급BALB／c소서수궤분위정상조、모형조、지새미송간예조、동강편조、익기고본효낭고제량조화익기고본효낭저제량조，매조10지。제정상조외，기여각조균이란청백단백치민화격발복제효천모형，우제21천기，재매차격발전1 h분별급여모형조화용약각조이순정수급약물관위，정상조불급여임하처치。응용HE염색관찰각조소서기도급폐조직적병이학개변，면역조직화학법검측폐조직TGF‐β1화P‐Smad3단백적표체。결과소서기도병리절편관찰모형조적기관、폐조직병리개변정도고우정상조、지새미송간예조、동강편조、익기고본효낭고제량조、익기고본효낭저제량조，차이유통계학의의（P＜0．05）；지새미송간예조여익기고본효낭고제량조、익기고본효낭저제량조상비，차이무통계학의의（P＞0．05）；여정상조상비，TGF‐β1단백재모형조、지새미송간예조、동강편조、익기고본효낭고제량조、익기고본효낭저제량조표체수평증강，차이유통계학의의（P＜0．01）；여모형조상비，지새미송간예조、동강편조、익기고본효낭고제량조、익기고본효낭저제량조TGF‐β1단백표체수평강저，차이유통계학의의（P＜0．01）；지새미송간예조여익기고본효낭고제량조、익기고본효낭저제량조상비，차이균무통계학의의（P＞0．05）；익기고본효낭고제량조여익기고본효낭저제량조상비，차이무통계학의의（P＞0．05）；여정상조상비，P‐Smad3단백재모형조、지새미송간예조、동강편조、익기고본효낭고제량조、익기고본효낭저제량조표체수평증강，차이유통계학의의（P＜0．01）；여모형조상비，지새미송간예조、동강편조、익기고본효낭고제량조、익기고본효낭저제량조P‐Smad3단백표체강저，차이유통계학의의（P＜0．01）；지새미송간예조 P‐Smad3단백표체비동강편조、익기고본효낭고제량조、익기고본효낭저제량조경저，차이유통계학의의（P＜0．01）；동강편조여익기고본효낭고제량조상비，차이무통계학의의（P＞0．05）；익기고본효낭고제량조여익기고본효낭저제량조상비，차이무통계학의의（P＞0．05）。결론익기고본효낭가억제효천소서체내TGF‐β1화P‐Smad3적과도표체，종이조단TGF‐β1포내신호전도，가재일정정도상감경효천소서기도염증화억제기도중소적발생발전。
Objective To observe the effects of different doses of Yiqi Guben capsules on pathology and TGF‐β1/Smad signaling pathway in airway remodeling of asthmatic mice .Method The 60 male SPF level BALB/c mice were randomly divided into normal group ,model group ,dexamethasone intervention(DSMS) group ,Tongkang (TKP) group ,Yiqi Guben capsules high dose(YQGBH) group and Yiqi Guben capsules low dose(YQGBL) group ,with 10 mice in each group .Apart from the normal group ,the other groups were sensitized and stimulated by ovalbumin to copy asthma model .On and from the 21st day , each group was given gavage with pure water and medicines 1 h prior to each excitation except the normal group .Observe path‐ological changes of airway and lung tissues in each group with HE staining ,and detect expression of TGF‐β1 and P‐Smad3 pro‐tein in lung tissues with immunohistochemistry .Result The trachea and lung tissue pathological change degree in model group was statistically higher than that of normal group ,model group ,DSMS group ,TKP group ,YQGBH group and YQGBL group according to airway pathological examination(P<0 .05);comparing DSMS group with YQGBH group and YQGBL group ,the difference was not statistically significant(P>0 .05) .Compared with normal group ,TGF‐β1 protein expression levels in model group ,DSMS group ,TKP group ,YQGBH group and YQGBL group statistically increased(P<0 .01);compared with model group ,TGF‐β1 protein expression levels statistically decreased in DSMS group ,TKP group ,YQGBH group and YQGBL group(P<0 .01);comparing DSMS group with YQGBH group and YQGBL group ,there was no statistical significance(P>0 .05);comparing YQGBH group with YQGBL group ,the difference was not statistically significant(P>0 .05);compared with normal group ,the expression levels of P‐Smad3 protein increased in DSMS group ,TKP group ,YQGBH group and YQGBL group(P<0 .01);compared with model group ,the P‐Smad3 protein expression levels decreased in DSMS group ,TKP group , YQGBH group and YQGBL group with statiscal significance(P<0 .01);the P‐Smad3 protein expression level of DSMS group was even lower than TKP group ,YQGBH group and YQGBL group(P<0 .01) and the difference was of statistical signifi‐cance;comaring TKP group with YQGBH group ,the difference was not statistically significant(P>0 .05);comparing YQGBH group with YQGBL group ,there was no statistically significant difference(P>0 .05) .Conclusion Yiqi Guben capsules can block TGF‐β1 intracellular signal transduction by inhibiting excessive expression of TGF‐β1 and P‐Smad3 in asthma mice ,which to a certain extent ,reduces asthma airway inflammation in mice and inhibits the occurrence of airway remodeling .