CAJ | 학술논문

为了筛选出高产油菌株，本实验采用细胞形态学与细胞化学方法（苏丹黑染色法）对常现青霉P1、土壤青霉P3、绿色木霉L1、红色脉孢霉H1四株霉菌进行跟踪观察，通过在不同葡萄糖浓度梯度的发酵液中摇瓶培养，并分析菌种生物量、葡萄糖利用率和油脂含量，从而确定最经济有效的葡萄糖浓度。结果表明，高浓度的葡萄糖会抑制霉菌油脂的合成，L1菌株在培养基中初始葡萄糖浓度为4%时，积累生物量、葡萄糖转化率均较大，油脂产量最佳为2.598g/L，菌体油含量达到38.62%（细胞干重）。
위료사선출고산유균주，본실험채용세포형태학여세포화학방법（소단흑염색법）대상현청매P1、토양청매P3、록색목매L1、홍색맥포매H1사주매균진행근종관찰，통과재불동포도당농도제도적발효액중요병배양，병분석균충생물량、포도당이용솔화유지함량，종이학정최경제유효적포도당농도。결과표명，고농도적포도당회억제매균유지적합성，L1균주재배양기중초시포도당농도위4%시，적루생물량、포도당전화솔균교대，유지산량최가위2.598g/L，균체유함량체도38.62%（세포간중）。
We used four strains(Penicillium flavidorsum P1、Penicillium terrestre P3、Trichoderma viride L1、Neuro?sporspp H1)to screen out the high lipid production strains. We adopted cell morphology and cytochemical methods (Sudan Black dyeing technique)to observe intracellular characteristics. Culture the four strains in fermentation solu?tions with different glucose concentrations,and determine the most economic glucose concentration through the analysis of bacteria biomass,glucose utilization and fat content. Observa?tion results indicated that glucose with high concentrations would inhibit the synthesis of fungal oils,L1 strain had a great accumulation of biomass and glucose conversion rate when the concentration of glucose of the media for fermen?tation is 4%,with the lipid yield up to 2.598g/L,and the lipid concentration reach 38.62%(W/try cell).