世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2015年
6期
1150-1156
,共7页
周静鑫%孙文%张岩%吴丽丽%秦灵灵%郭璇%许光远%李林忆%段颖%郭翔宇%吴欣莉%刘铜华
週靜鑫%孫文%張巖%吳麗麗%秦靈靈%郭璇%許光遠%李林憶%段穎%郭翔宇%吳訢莉%劉銅華
주정흠%손문%장암%오려려%진령령%곽선%허광원%리림억%단영%곽상우%오흔리%류동화
芪卫颗粒%糖尿病肾病%足细胞损伤%KK-Ay小鼠
芪衛顆粒%糖尿病腎病%足細胞損傷%KK-Ay小鼠
기위과립%당뇨병신병%족세포손상%KK-Ay소서
Qiwei granules%diabetic nephropathy%podocyte injury%KK-Ay mice
目的：探讨芪卫颗粒干预糖尿病肾病小鼠足细胞损伤的作用机制。方法：28只8周龄KK-Ay小鼠成模后分为模型组和芪卫低剂量组、芪卫中剂量组及芪卫高剂量组，另8只C57BL/6J小鼠设为正常对照组。实验中纪录小鼠一般状况、血糖、24 h尿蛋白定量。治疗10周后检测小鼠肾功能指标血清肌酐及尿素氮，并称取小鼠肾脏，固定后行小鼠肾组织苏木精-伊红（HE）、Masson、过碘酸希夫（PAS）染色，并制作肾母细胞瘤基因（WT-1）免疫组化，结合软件分析计算小鼠肾小球足细胞数。蛋白质印迹法（Western Blot）检测小鼠肾脏中nephrin蛋白表达，实时荧光定量聚合酶链反应（PCR）检测nephrin mRNA的表达。结果：与模型组相比，芪卫颗粒治疗10周后小鼠体质量、血糖、24 h尿蛋白定量及血肌酐明显降低，并有效改善肾小球系膜增生等病理损害，保护足细胞数目。芪卫颗粒治疗后的nephrin蛋白表达量及mRNA表达量均显高于模型组。结论：芪卫颗粒可能通过调节nephrin表达而改善糖尿病肾病小鼠足细胞损伤。
目的：探討芪衛顆粒榦預糖尿病腎病小鼠足細胞損傷的作用機製。方法：28隻8週齡KK-Ay小鼠成模後分為模型組和芪衛低劑量組、芪衛中劑量組及芪衛高劑量組，另8隻C57BL/6J小鼠設為正常對照組。實驗中紀錄小鼠一般狀況、血糖、24 h尿蛋白定量。治療10週後檢測小鼠腎功能指標血清肌酐及尿素氮，併稱取小鼠腎髒，固定後行小鼠腎組織囌木精-伊紅（HE）、Masson、過碘痠希伕（PAS）染色，併製作腎母細胞瘤基因（WT-1）免疫組化，結閤軟件分析計算小鼠腎小毬足細胞數。蛋白質印跡法（Western Blot）檢測小鼠腎髒中nephrin蛋白錶達，實時熒光定量聚閤酶鏈反應（PCR）檢測nephrin mRNA的錶達。結果：與模型組相比，芪衛顆粒治療10週後小鼠體質量、血糖、24 h尿蛋白定量及血肌酐明顯降低，併有效改善腎小毬繫膜增生等病理損害，保護足細胞數目。芪衛顆粒治療後的nephrin蛋白錶達量及mRNA錶達量均顯高于模型組。結論：芪衛顆粒可能通過調節nephrin錶達而改善糖尿病腎病小鼠足細胞損傷。
목적：탐토기위과립간예당뇨병신병소서족세포손상적작용궤제。방법：28지8주령KK-Ay소서성모후분위모형조화기위저제량조、기위중제량조급기위고제량조，령8지C57BL/6J소서설위정상대조조。실험중기록소서일반상황、혈당、24 h뇨단백정량。치료10주후검측소서신공능지표혈청기항급뇨소담，병칭취소서신장，고정후행소서신조직소목정-이홍（HE）、Masson、과전산희부（PAS）염색，병제작신모세포류기인（WT-1）면역조화，결합연건분석계산소서신소구족세포수。단백질인적법（Western Blot）검측소서신장중nephrin단백표체，실시형광정량취합매련반응（PCR）검측nephrin mRNA적표체。결과：여모형조상비，기위과립치료10주후소서체질량、혈당、24 h뇨단백정량급혈기항명현강저，병유효개선신소구계막증생등병리손해，보호족세포수목。기위과립치료후적nephrin단백표체량급mRNA표체량균현고우모형조。결론：기위과립가능통과조절nephrin표체이개선당뇨병신병소서족세포손상。
This article was aimed to study the effect ofQiwei granules on the podocyte in KK-Ay mice kidney. The 28 8-week-old male KK-Ay mice were randomly divided into the model group, low-dosage, middle-dosage and high-dosageQiwei granule group. Eight C57BL/6J mice were used as the normal control. The general conditions, blood glucose and 24 h albuminuria were recorded in the experiment. After 10-week treatment, renal indexes including serum creatinine and urea nitrogen were measured. The kidneys of mice were collected and measured. The hematoxylin and eosin (HE), Masson’s Trichrome, and periodic acid-Schiff (PAS) were used on renal tissues of mice. The immunohistochemical staining for WT-1 was made. Software analysis was combined in the calculation of renal podocyte amount. Western blot was used in the detection of nephrin protein expressions in the kidney of mice. RT-PCR was used in the detection of nephrin mRNA expression. The results showed that compared with the model group, the body weight, blood glucose, 24 h albuminuria and the serum creatinine were obviously decreased after 10-week treatment ofQiwei granules. It can effectively improve the glomerular mesangial proliferation and preserve the podocyte number. Meanwhile, after the treatment ofQiwei granules, the nephrin protein expression and mRNA expression were obviously higher than the model group. It was concluded thatQiwei granules probably managed nephrin expression to improve the podocyte injury in the diabetic nephropathy of KK-Ay mice.
