CAJ | 학술논문

目的：探讨乙肝病毒变异检测中基因芯片技术的临床应用价值，为临床乙肝疾病诊治提供参考依据。方法选择2014年确诊为慢性乙肝疾病的患者200例作为研究对象，使用基因芯片技术测定研究对象的血清中乙肝病毒前 C 区 A1896、A1814以及 BCP 区 nt1762、nt1764的变异情况，对比患者临床诊断分型、乙肝病毒 DNA 复制程度和血清 e 系统进行综合分析。结果200例研究对象中，乙肝病毒前 C 区 A1896、A1814、BCP 区 nt1762和 BCP 区nt1764变异阳性率分别为58．5％、13．0％、55．0％、53．0％，且随着患者病情加重，乙肝病毒的变异情况发生情况逐步增多；HBeAg（-）HBeAb（＋）患者或乙肝病毒 DNA 定量在104-106 copy／ml 之间时乙肝病毒变异发生率最高．结论使用基因芯片技术检测乙肝病毒多位点变异情况对临床疾病的诊治具有一定的应用价值，值得推广。
목적：탐토을간병독변이검측중기인심편기술적림상응용개치，위림상을간질병진치제공삼고의거。방법선택2014년학진위만성을간질병적환자200례작위연구대상，사용기인심편기술측정연구대상적혈청중을간병독전 C 구 A1896、A1814이급 BCP 구 nt1762、nt1764적변이정황，대비환자림상진단분형、을간병독 DNA 복제정도화혈청 e 계통진행종합분석。결과200례연구대상중，을간병독전 C 구 A1896、A1814、BCP 구 nt1762화 BCP 구nt1764변이양성솔분별위58．5％、13．0％、55．0％、53．0％，차수착환자병정가중，을간병독적변이정황발생정황축보증다；HBeAg（-）HBeAb（＋）환자혹을간병독 DNA 정량재104-106 copy／ml 지간시을간병독변이발생솔최고．결론사용기인심편기술검측을간병독다위점변이정황대림상질병적진치구유일정적응용개치，치득추엄。
Objective To explore the clinical value of the Hepatitis B virus mutation detection by the gene chip tech-nology testing,guiding the clinical diagnosis and treatment.Methods Choosing 200 cases of patientes with the chronic hepatitis B disease for the stuy in 2014,testing the Pre-C A1896,Pre-C A1814,BCP area nt1762,BCP area nt1764 of the Hepatitis B virus mutation by the gene chip technology,comparative clinical diagnostic typing,hepatitis B virus DNA replication degree and a comprehensive analysis of serum e system.Results For the 200 cases of patientes with the chronic hepatitis B disease,the positive rate of the Pre-C A1896,Pre-C A1814,BCP area nt1762,BCP area nt1764 of the Hepatitis B virus mutation was 58.5%,13.0%,55.0% and 53.0%,and with sicker patients,variations in the inci-dence of hepatitis B virus gradually increased;the highest incidence of hepatitis B virus mutates when the patient was HBeAg(-)HBeAb(+)and HBV DNA quantitation between 104-106 copy/ml.Conclusion There is a certain value for the clinical diagnosis and treatment by the gene chip technology testing of the many point variation of hepatitis B virus, and worthy to promot.