CAJ | 학술논문

目的：建立一种简单、快速的核酸检测新方法，即环介导等温扩增方法（LAMP）来检测人细小病毒 B19的核酸。方法从 GenBank 中获得 B19病毒 VP2基因序列，利用 PrimerExplorer V3软件在序列保守区域设计 LAMP引物，同时对试验中的几个重要参教进行优化，并评价其敏感性和特异性。结果 LAMP 检测方法的敏感性高于PCR 方法，并且对 B19病毒的检测具有特异性。结论建立了环介导等温扩增快速检测方法，此方法灵敏性高、特异性强，不仅适用于实验室检测，更能够广泛用于临床检测。
목적：건립일충간단、쾌속적핵산검측신방법，즉배개도등온확증방법（LAMP）래검측인세소병독 B19적핵산。방법종 GenBank 중획득 B19병독 VP2기인서렬，이용 PrimerExplorer V3연건재서렬보수구역설계 LAMP인물，동시대시험중적궤개중요삼교진행우화，병평개기민감성화특이성。결과 LAMP 검측방법적민감성고우PCR 방법，병차대 B19병독적검측구유특이성。결론건립료배개도등온확증쾌속검측방법，차방법령민성고、특이성강，불부괄용우실험실검측，경능구엄범용우림상검측。
Objective To establish a simple and rapid new method for nucleic acid detection,loop-mediated isother-mal amplification (LAMP)to detect human parvovirus B19 nucleic acid.Methods B19 virus of VP2 gene was down-loaded from the GenBank,then primers of LAMP designed using PrimerExplorer V3 software from conserved regions, while the test of several important parameters to optimize teaching and evaluation of its sensitivity and specificity.Re-sults LAMP method is more sensitive than PCR detection method,and specific on the detection of B19.Conclusion Loop-mediated isothermal amplification rapid detection methods has high sensitivity and specificity,not only suitable for laboratory testing,but can be widely used in clinical testing.