中国临床神经科学
中國臨床神經科學
중국림상신경과학
CHINESE JOURNAL OF CLINICAL NEUROSCIENCES
2015年
4期
399-405
,共7页
罗家明%谭书伟%陈蓉%王莉%柯莎%蒋国会%刘超%苟婷%余巨明%秦新月
囉傢明%譚書偉%陳蓉%王莉%柯莎%蔣國會%劉超%茍婷%餘巨明%秦新月
라가명%담서위%진용%왕리%가사%장국회%류초%구정%여거명%진신월
实验性自身免疫性脑脊髓炎%多发性硬化%基质金属蛋白酶-9%芬戈莫德%细胞外基质%血脑屏障
實驗性自身免疫性腦脊髓炎%多髮性硬化%基質金屬蛋白酶-9%芬戈莫德%細胞外基質%血腦屏障
실험성자신면역성뇌척수염%다발성경화%기질금속단백매-9%분과막덕%세포외기질%혈뇌병장
experimental autoimmune encephalomyelitis%multiple sclerosis%matrix metalloproteinase-9
目的：探讨芬戈莫德对实验性自身免疫性脑脊髓炎(EAE)小鼠脑组织基质金属蛋白酶-9(MMP-9)mRNA及蛋白表达的影响。方法48只雌性C57BL/6小鼠随机分为完全弗氏佐剂(CFA)组、EAE组、芬戈莫德组。运用髓鞘少突胶质细胞糖蛋白35-55构建EAE小鼠模型，小鼠神经功能缺损评分达到1分或免疫第14天，芬戈莫德组腹腔注射芬戈莫德10 mg·kg-1，相应的CFA组、EAE组给予生理盐水。观察小鼠行为学变化，中枢炎症和脱髓鞘情况，脑组织中的MMP-9 mRNA及蛋白表达。结果芬戈莫德组小鼠临床症状减轻，体重下降减少，潜伏期延长，发病率降低，炎性病灶数减少，脱髓鞘程度减轻。芬戈莫德组MMP-9 mRNA和蛋白表达水平较EAE组降低。结论芬戈莫德能抑制EAE小鼠脑组织中MMP-9 mRNA及蛋白表达水平。
目的：探討芬戈莫德對實驗性自身免疫性腦脊髓炎(EAE)小鼠腦組織基質金屬蛋白酶-9(MMP-9)mRNA及蛋白錶達的影響。方法48隻雌性C57BL/6小鼠隨機分為完全弗氏佐劑(CFA)組、EAE組、芬戈莫德組。運用髓鞘少突膠質細胞糖蛋白35-55構建EAE小鼠模型，小鼠神經功能缺損評分達到1分或免疫第14天，芬戈莫德組腹腔註射芬戈莫德10 mg·kg-1，相應的CFA組、EAE組給予生理鹽水。觀察小鼠行為學變化，中樞炎癥和脫髓鞘情況，腦組織中的MMP-9 mRNA及蛋白錶達。結果芬戈莫德組小鼠臨床癥狀減輕，體重下降減少，潛伏期延長，髮病率降低，炎性病竈數減少，脫髓鞘程度減輕。芬戈莫德組MMP-9 mRNA和蛋白錶達水平較EAE組降低。結論芬戈莫德能抑製EAE小鼠腦組織中MMP-9 mRNA及蛋白錶達水平。
목적：탐토분과막덕대실험성자신면역성뇌척수염(EAE)소서뇌조직기질금속단백매-9(MMP-9)mRNA급단백표체적영향。방법48지자성C57BL/6소서수궤분위완전불씨좌제(CFA)조、EAE조、분과막덕조。운용수초소돌효질세포당단백35-55구건EAE소서모형，소서신경공능결손평분체도1분혹면역제14천，분과막덕조복강주사분과막덕10 mg·kg-1，상응적CFA조、EAE조급여생리염수。관찰소서행위학변화，중추염증화탈수초정황，뇌조직중적MMP-9 mRNA급단백표체。결과분과막덕조소서림상증상감경，체중하강감소，잠복기연장，발병솔강저，염성병조수감소，탈수초정도감경。분과막덕조MMP-9 mRNA화단백표체수평교EAE조강저。결론분과막덕능억제EAE소서뇌조직중MMP-9 mRNA급단백표체수평。
Aim To investigate the effect of fingolimod (FTY720) on the expression of matrix metalloproteinase-9 (MMP-9) in the brain tissue of experimental autoimmune encephalomyelitis (EAE). Methods Forty-eight C57BL/6 mice were randomly divided into three groups equally:EAE group (made by using myelin oligodendrocyte glycoprotein (MOG35-55), treated with normal saline), CFA group (made by using normal saline instead of MOG35-55, treated with normal saline), FTY720 group (made as EAE group, intraperitoneal injected FTY720 (10 mg·kg-1). Mice were examined daily for clinical signs and scored according to the signs and symptoms. Hematoxylin-eosin staining and Luxol Fast Blue myelin staining were performed on the sections of brain. The number of inflammation focus in the brain tissue was counted under the optical microscope. The expressions of MMP-9 in brain tissue were detected by immunohistochemistry technique, while mRNA expression of MMP-9 in mice were detected by real-time PCR technology. Results Comparing to EAE group, neurological deifcit scores, weight 1oss, eclipse period and morbidity decreased in FTY720 group, as well as the amount of the inlfammation focus and demyelination lesions. The expression level of MMP-9 and MMP-9 mRNA in FTY720 group were lower than that of EAE group. Conclusion FTY720 inhibit the expression level of MMP-9 protein and mRNA in the brain tissue of EAE mice.
