CAJ | 학술논문

目的：研究miRNA-449基因表达水平与神经胶质瘤的发生的相关性分析。方法选择神经胶质瘤患者66例(胶质瘤组)，胶质瘤组再按分级分为2个亚组：低级别胶质瘤组(30例)；高级别胶质瘤组(36例)。另选择颅内减压术脑外伤患者的正常脑组织标本10例为对照组。提取脑组织，经实时定量PCR扩增进行荧光定量分析miRNA-449基因表达，采用MS-PCR方法检测miRNA-449甲基化水平。结果定量PCR检测结果显示：对照组患者脑组织miRNA-449基因表达水平高于胶质瘤组(P=0.000)；高级别胶质瘤组miRNA-449基因表达水平低于低级别胶质瘤组(P=0.044)。对66例脑胶质瘤患者miRNA-449基因启动子区甲基化水平进行检测，并与其表达水平进行相关性分析，发现在高甲基化患者miRNA-449基因表达水平低于无甲基化患者(P=0.0002)。结论 miRNA-449基因表达降低可能与miRNA-449基因启动子区甲基化水平有一定相关性，且可能参与神经胶质瘤的发生发展，为疾病进展的早期监测提供分子理论依据。
목적：연구miRNA-449기인표체수평여신경효질류적발생적상관성분석。방법선택신경효질류환자66례(효질류조)，효질류조재안분급분위2개아조：저급별효질류조(30례)；고급별효질류조(36례)。령선택로내감압술뇌외상환자적정상뇌조직표본10례위대조조。제취뇌조직，경실시정량PCR확증진행형광정량분석miRNA-449기인표체，채용MS-PCR방법검측miRNA-449갑기화수평。결과정량PCR검측결과현시：대조조환자뇌조직miRNA-449기인표체수평고우효질류조(P=0.000)；고급별효질류조miRNA-449기인표체수평저우저급별효질류조(P=0.044)。대66례뇌효질류환자miRNA-449기인계동자구갑기화수평진행검측，병여기표체수평진행상관성분석，발현재고갑기화환자miRNA-449기인표체수평저우무갑기화환자(P=0.0002)。결론 miRNA-449기인표체강저가능여miRNA-449기인계동자구갑기화수평유일정상관성，차가능삼여신경효질류적발생발전，위질병진전적조기감측제공분자이론의거。
Aim To evaluate the signiifcance of miRNA-449 expression in glioblastoma. Methods Sixty six patients with glioblastoma and 10 healthy donors were adopted in this study. RNA was isolated from cerebra tissue, and quantitative real-time PCR (Q-PCR) were used to detect the levels of miRNA-449 gene in health donors and newly diagnosed glioblastoma patients. Results Q-PCR analysis showed that the levels of miRNA-449 in glioblastoma group were lower than those in healthy donors group (P=0.000). Furthermore, the miRNA-449 expression was lower in high stage disease than that in low stage group (P=0.044). The methylation levels of the miRNA-449 was detected by MS-PCR. The levels of miRNA-449 in high methylation group were found to be lower than that in low methylation group (P=0.000 2). Conclusion The aberrant expression of the miRNA-449 gene was perhaps correlated with the levels of miRNA-449 methylation and may be involved in the occurrence and progression of glioblastoma, which provided the molecular basis of the early monitoring disease progression.