CAJ | 학술논문

目的：：探讨姜黄素( Cur）对人结肠癌耐奥沙利铂( Oxa）细胞株( SW620／OxR）的逆转耐药作用及其机制是否与抑制STAT3信号通路有关。方法：采用WST-1试剂检测Cur对SW620／OxR细胞株的半数抑制( IC50）浓度，选择IC50低一浓度梯度的Cur＋2μmol／L Oxa作用于SW620／OxR细胞48 h(称为实验组），与仅加入2μmol／L的Oxa对照组比较，①采用流式细胞术检测细胞凋亡；②采用Western blot检测STAT3活化标志磷酸化STAT3( P-STAT3）蛋白及其下游耐药相关靶分子P糖蛋白( P-gp）的表达情况。结果：Cur对SW620／OxR细胞的IC50浓度为18.9μmol／L；实验组与对照组比较：①细胞凋亡率由(5.08±1.82）％上升到(30.69±2.94）％，实验组细胞凋亡率明显高于对照组(P＜0.05）；②P-STAT3及P-gp表达量均明显受抑制( P＜0.05）。结论：Cur具有调控人结肠癌耐药细胞株耐药性的作用，可能与抑制STAT3信号通路，降低P-gp表达有关。
목적：：탐토강황소( Cur）대인결장암내오사리박( Oxa）세포주( SW620／OxR）적역전내약작용급기궤제시부여억제STAT3신호통로유관。방법：채용WST-1시제검측Cur대SW620／OxR세포주적반수억제( IC50）농도，선택IC50저일농도제도적Cur＋2μmol／L Oxa작용우SW620／OxR세포48 h(칭위실험조），여부가입2μmol／L적Oxa대조조비교，①채용류식세포술검측세포조망；②채용Western blot검측STAT3활화표지린산화STAT3( P-STAT3）단백급기하유내약상관파분자P당단백( P-gp）적표체정황。결과：Cur대SW620／OxR세포적IC50농도위18.9μmol／L；실험조여대조조비교：①세포조망솔유(5.08±1.82）％상승도(30.69±2.94）％，실험조세포조망솔명현고우대조조(P＜0.05）；②P-STAT3급P-gp표체량균명현수억제( P＜0.05）。결론：Cur구유조공인결장암내약세포주내약성적작용，가능여억제STAT3신호통로，강저P-gp표체유관。
Objective:To investigate the reversal multidrug resistance effects of curcumin on human colorectal cancer cell lines resistant to oxaliplatin( SW620/OxR) and whether its mechanism was involved in downregulation of STAT3 signaling.Methods: The IC50 value(50%cell growth inhibitory concentrations) of curcumin to SW620/OxR cell lines was obtained by WST-1 reagent,which was one kind of cell proliferation assay.Later experiments included in the experimental group and the control group.In the experimental group,SW620/OxR cell lines were exposed to the previous experiment IC50 concentrations of curcumin plus 2 μmol/L oxaliplatin for 48 h,while in the control group,SW620/OxR cell lines were cultured in medium containing in 2μmol/L oxaliplatin.In the two groups:apoptosis was detected by flow cytometry;the protein expression levels of phosphorylated STAT3(P-STAT3) and P-gp were disclosed by Western blot.Results:The IC50 value of curcumin to SW620/OxR cell lines was 18.9 μmol/L.The apoptosis rate of cells in the control group and the experimental group were respectively ( 5.08 ±1.82 )% and ( 30.69 ±2.94 )%, the apoptosis rate of the experimental group was significantly higher than that of the control group ( P<0.05 ) .The protein expression levels of phosphorylated STAT3(P-STAT3) and P-gp in the experimental group was significantly lower than that in the control group(P<0.05).Conclusion:Curcumin can reverse drug resistance in colorectal cancer cell lines resistant to oxaliplatin, its effect may be associated with downregulation of STAT3 signaling pathways.