CAJ | 학술논문

目的：观察消黄方对异硫氰酸α-萘酯(ANIT)诱导胆汁淤积大鼠的肝保护作用。方法将40只Wistar雄性大鼠按随机区组法分为空白对照组、模型组、消黄方组、熊去氧胆酸组各10只。除空白对照组外，其余各组大鼠灌胃ANIT溶液6 ml/kg制备大鼠胆汁淤积性黄疸模型。ANIT灌胃48 h后，消黄方组大鼠灌胃消黄方药液1.73 g/kg，熊去氧胆酸组灌胃熊去氧胆酸胶囊溶液10 mg/kg，空白对照组与模型组灌胃等体积生理盐水。给药7 d后取材。检测各组大鼠血清肝功能指标，采用HE染色观察各组大鼠肝组织病理学改变，采用免疫组化染色和免疫蛋白印迹技术检测CD68表达。结果与模型组比较，消黄方组大鼠谷氨酸转氨酶(ALT)[(164.6±53.4)U/L 比(208.4±28.5)U/L]、天冬氨酸转氨酶(AST)[(247.6±76.1)U/L 比(341.8±32.8)U/L]、碱性磷酸酶(ALP)[(601.0±101.1)U/L 比(720.6±123.3)U/L]、总胆红素(TBiL)[(96.5±18.1)μmol/L 比(149.6±30.2)μmol/L]、直接胆红素(DBiL)[(73.7±16.6)μmol/L比(140.3±28.6)μmol/L]、总胆汁酸(TBA)[(93.4±13.0)μmol/L比(146.5±38.9)μmol/L]水平均显著降低(P＜0.01或P＜0.05)。消黄方组大鼠肝组织CD68蛋白[(7.08±0.19)比(17.42±0.48)]表达较模型组降低(P＜0.01)。结论消黄方可改善肝功能指标，降低CD68蛋白表达，发挥保肝退黄作用。
목적：관찰소황방대이류청산α-내지(ANIT)유도담즙어적대서적간보호작용。방법장40지Wistar웅성대서안수궤구조법분위공백대조조、모형조、소황방조、웅거양담산조각10지。제공백대조조외，기여각조대서관위ANIT용액6 ml/kg제비대서담즙어적성황달모형。ANIT관위48 h후，소황방조대서관위소황방약액1.73 g/kg，웅거양담산조관위웅거양담산효낭용액10 mg/kg，공백대조조여모형조관위등체적생리염수。급약7 d후취재。검측각조대서혈청간공능지표，채용HE염색관찰각조대서간조직병이학개변，채용면역조화염색화면역단백인적기술검측CD68표체。결과여모형조비교，소황방조대서곡안산전안매(ALT)[(164.6±53.4)U/L 비(208.4±28.5)U/L]、천동안산전안매(AST)[(247.6±76.1)U/L 비(341.8±32.8)U/L]、감성린산매(ALP)[(601.0±101.1)U/L 비(720.6±123.3)U/L]、총담홍소(TBiL)[(96.5±18.1)μmol/L 비(149.6±30.2)μmol/L]、직접담홍소(DBiL)[(73.7±16.6)μmol/L비(140.3±28.6)μmol/L]、총담즙산(TBA)[(93.4±13.0)μmol/L비(146.5±38.9)μmol/L]수평균현저강저(P＜0.01혹P＜0.05)。소황방조대서간조직CD68단백[(7.08±0.19)비(17.42±0.48)]표체교모형조강저(P＜0.01)。결론소황방가개선간공능지표，강저CD68단백표체，발휘보간퇴황작용。
Objective To observe the protective effect of Xiaohuangdecotion against liver damage inα-naphthylisothiocyanate (ANIT)- induced cholestasis in rats and probe the potential mechanisms.Methods Male Wistar rats (40) were randomly divided into a normal group, a model group, aXiaohuangdecotion treatment group, and a UDCA control group (10 for each). Except for rats in the normal group, ANIT solution (6 ml/kg) was administered in other rats by gavages for cholestasis model. After ANIT treated 48 h, rats inXiaohuangdecotion group and UDCA group were treated withXiaohuangdecotion (1.73 g/kg) and UDCA (10 mg/kg) respectively for 1 week. And, rats in the normal group and the model group were given an equal volume of saline. At the end of the experiment, liver function rats were examined. Liver histology was examined by HE staining, and CD68 factor was tested by immunohistochemistry and Western blot.Results Compared with the model group, the content of ALT (164.6 ± 53.4 U/Lvs. 208.4 ± 28.5 U/L), AST (247.6 ± 76.1 U/Lvs. 341.8 ± 32.8 U/L), ALP (601.0 ± 101.1 U/Lvs. 720.6 ± 123.3 U/L), TBiL (96.5 ± 18.1μmol/Lvs. 149.6 ± 30.2μmol/L), DBiL (73.7 ± 16.6μmol/Lvs. 140.3 ± 28.6μmol/L) and TBA (93.4 ± 13.0μmol/Lvs. 146.5 ± 38.9μmol/L) were significantly reduced in the treatment group (P<0.01 orP<0.05). Compared with the model group, CD68 level (7.08 ± 0.19 vs. 17.42 ± 0.48)were significantly reduced by intervention ofXiaohuangdecotion (P<0.01).ConclusionsXiaohuangdecotion could improve liver functions and reduce CD68 expression, leading to a good hepatoprotective and jaundice-relieving effects.