CAJ | 학술논문

目的：：探索静水高压在研制黑色素瘤疫苗方面的应用。方法：使用静水高压、液氮反复冻融两种方式破碎鼠源性B16黑色素瘤细胞。将破碎后的细胞悬液与弗氏佐剂混合后制成疫苗用于免疫小鼠，免疫完成后，行尾静脉注射与皮下注射接种肿瘤。通过比较每组小鼠生存时间、皮下肿瘤体积大小、DTH实验、小鼠体内肿瘤荧光成像来比较每种疫苗的免疫效果。结果：相比于液氮冻融及对照组，静水高压组小鼠生存时间长，皮下肿瘤体积小，DTH实验与肿瘤荧光成像均有明显差别。结论：静水高压破碎细胞相比于液氮反复冻融破碎在研制肿瘤疫苗方面有优势。
목적：：탐색정수고압재연제흑색소류역묘방면적응용。방법：사용정수고압、액담반복동융량충방식파쇄서원성B16흑색소류세포。장파쇄후적세포현액여불씨좌제혼합후제성역묘용우면역소서，면역완성후，행미정맥주사여피하주사접충종류。통과비교매조소서생존시간、피하종류체적대소、DTH실험、소서체내종류형광성상래비교매충역묘적면역효과。결과：상비우액담동융급대조조，정수고압조소서생존시간장，피하종류체적소，DTH실험여종류형광성상균유명현차별。결론：정수고압파쇄세포상비우액담반복동융파쇄재연제종류역묘방면유우세。
Objective: To explore the application of high hydrostatic pressure in the development of melanoma vaccine.Methods:The high hydrostatic pressure,liquid nitrogen freezing and thawing were used to break the murine B16 melanoma cells and then the cell suspension was mixed with Freund's adjuvant to prepare vaccine for immunizing the mice.Results:After immu-nization,the murine B16 melanoma cells were injected intravenously and subcutaneously.The immune results of the vaccines were evaluated,by comparing survival time of the mice, subcutaneous tumor volume, DTH experiments and the fluorescence imaging of tumors.Conclusion:Compared with the method of liquid nitrogen freeze-thaw broken,high hydrostatic pressure crushing cells has more advantages in the development of tumor vaccine.