CAJ | 학술논문

目的：探讨表皮生长因子( epidermal growth factor, EGF)膜对皮肤切口愈合促进及瘢痕抑制的作用。方法选取36只SD大鼠随机将其分为治疗组和对照组各18只,麻醉后在脊柱两侧做皮肤切口至肌层,缝合伤口,对照组外敷空白膜,治疗组外敷自制EGF膜,每日更换,两组分别于伤后3、5、7、9、14、21 d各处死3只大鼠,制取标本,于肉眼下、Masson 3色染色及EGF免疫组织化学染色(伤后14、21 d未进行)后观察切口皮肤,并测定Ⅲ型前胶原( PCⅢ)含量。结果肉眼观察治疗组表皮层和真皮层愈合早于对照组。 masson 3色染色结果显示对照组胶原纤维粗大,与创口呈垂直状态；治疗组成纤维细胞排列疏松、规则,伤口两侧新生胶原纤维量较少,胶原束纤细,排列相对疏松。EGF免疫组织化学染色结果显示对照组伤后3、5 d表皮上层由阴性转向强阳性,伤后7 d表皮上层与伤后5 d比较变化不明显,伤后9d表皮上层呈阴性；治疗组伤后3、5、7、9d表皮上层呈强阳或阳性。伤后3、5、7、9、14dPCⅢ含量治疗组均高于对照组,差异有统计学意义(P<0．05)。结论应用外源性EGF能提高伤口中EGF水平,可促进伤口愈合,抑制瘢痕形成。
목적：탐토표피생장인자( epidermal growth factor, EGF)막대피부절구유합촉진급반흔억제적작용。방법선취36지SD대서수궤장기분위치료조화대조조각18지,마취후재척주량측주피부절구지기층,봉합상구,대조조외부공백막,치료조외부자제EGF막,매일경환,량조분별우상후3、5、7、9、14、21 d각처사3지대서,제취표본,우육안하、Masson 3색염색급EGF면역조직화학염색(상후14、21 d미진행)후관찰절구피부,병측정Ⅲ형전효원( PCⅢ)함량。결과육안관찰치료조표피층화진피층유합조우대조조。 masson 3색염색결과현시대조조효원섬유조대,여창구정수직상태；치료조성섬유세포배렬소송、규칙,상구량측신생효원섬유량교소,효원속섬세,배렬상대소송。EGF면역조직화학염색결과현시대조조상후3、5 d표피상층유음성전향강양성,상후7 d표피상층여상후5 d비교변화불명현,상후9d표피상층정음성；치료조상후3、5、7、9d표피상층정강양혹양성。상후3、5、7、9、14dPCⅢ함량치료조균고우대조조,차이유통계학의의(P<0．05)。결론응용외원성EGF능제고상구중EGF수평,가촉진상구유합,억제반흔형성。
Objective To study the effect of promoting healing and scar inhibition of epidermal growth factor ( EGF) film on the dermal wound healing of rats. Methods 36 SD rats were randomly divided into treatment group and control group ( n=18 each group) . Under general anesthesia, two dermal incisions on both sides of the spine were as deep as the muscle layers. The incisions of two groups were applied with black film and epidermal growth factor ( EGF) film respectively. All films were changed daily to avoid infection. For each group three rats were killed on 3, 5, 7, 9, 14, 21 day after surgery and samples were obtained. The dermal incision was observed by visual and the histological study. In brief, incision changes were investigated by masson's trichrome staining and the expression of EGF in skin was detected with Streptavidin Peroxidase ( SP) immunohistochemical methods (not performed 14, 21 d after injury ). The content of PCⅢ was also determined. Results The healing velocity of epidermal and dermis wounds were quicker in treatment group than that in control group. In terms of masson's trichrome staining:the new collagen fibers displayed smaller quantity, slimmer and looser arrangement in treatment group compared with that of control group. The epidermal of control group showed positive EGF staining on the 5th and 7th day, negative at the 3rd and 9th day. However, in treatment group, the upper part of the epidermal showed stronger positive EGF staining on the 3rd, 5th, 7th, 9th day. The content of PCⅢ in EGF film group was higher than that of the control group on all the days, except on the 21st day (P<0. 05). Conclusion The film made from EGF can enhance the content of EGF in wounds, improve wound healing process and repress scar. formation.