CAJ | 학술논문

在酸性2-吗啉乙磺酸介质中，建立基于纳米金表面等离子体的微量过氧化氢（ H2 O2）的比色检测体系，探讨裸眼检测途径下的分辨极限，提高体系稳定性并利用光谱分析精确定量H2 O2浓度（ c ）．结果表明，当c＜100μmol／L时，纳米金为团聚态，呈现蓝色；c＞120μmol／L时，纳米金为分散态，呈现红色．该方法的裸眼检测极限可分辨浓度差为20μmol／L．反应溶液的显色结果并不稳定，在反应后45 min内变化较快，c为60～100μmol／L的反应溶液也将逐渐由蓝色变为红色．溶液在570 nm处的吸光度（ OD570）检测证明，反应体系在检测后3 h内仍持续变化．反应10 min后，加入适量半胱甘肽终止反应，可使反应溶液的显色结果稳定，提高体系稳定性．实验测定不同浓度H2 O2反应产物的吸收光谱发现，c＜100μmol／L的反应孔产物在波长约550 nm处有吸收峰，且吸收强度与c成正相关．而c＞120μmol／L的反应产物吸收峰逐渐往短波长方向偏移，最终峰值约为540 nm．分析反应体系吸收光谱结果证明，各反应产物在630 nm和545 nm处吸光度的比值（ OD630／545）与c呈良好的线性关系．实验对c为100～120μmol／L的H2 O2溶液进行检测，定量浓度差为2μmol／L的H2 O2溶液，降低裸眼检测途径下该方法的分辨极限，为微量样品检测提供更好的平台．
재산성2-마람을광산개질중，건립기우납미금표면등리자체적미량과양화경（ H2 O2）적비색검측체계，탐토라안검측도경하적분변겁한，제고체계은정성병이용광보분석정학정량H2 O2농도（ c ）．결과표명，당c＜100μmol／L시，납미금위단취태，정현람색；c＞120μmol／L시，납미금위분산태，정현홍색．해방법적라안검측겁한가분변농도차위20μmol／L．반응용액적현색결과병불은정，재반응후45 min내변화교쾌，c위60～100μmol／L적반응용액야장축점유람색변위홍색．용액재570 nm처적흡광도（ OD570）검측증명，반응체계재검측후3 h내잉지속변화．반응10 min후，가입괄량반광감태종지반응，가사반응용액적현색결과은정，제고체계은정성．실험측정불동농도H2 O2반응산물적흡수광보발현，c＜100μmol／L적반응공산물재파장약550 nm처유흡수봉，차흡수강도여c성정상관．이c＞120μmol／L적반응산물흡수봉축점왕단파장방향편이，최종봉치약위540 nm．분석반응체계흡수광보결과증명，각반응산물재630 nm화545 nm처흡광도적비치（ OD630／545）여c정량호적선성관계．실험대c위100～120μmol／L적H2 O2용액진행검측，정량농도차위2μmol／L적H2 O2용액，강저라안검측도경하해방법적분변겁한，위미량양품검측제공경호적평태．
A colorimetric method was developed for the determination of trace amount of H2 O2 in acidic 2-( N-mor-pholino) ethanesulphonic acid buffer medium, based on surface plasma of gold nanoparticles.The detecting sys-tem, whose limit of detecting resolution with naked eyes was measured previously, was used to accurately quantify the concentration( c) of H2 O2 by spectral analysis after stabilization enhancement.The results show that blue-col-oured solutions with aggregated gold nanoparticles could be obtained when c <100 μmol/L, while the solutions were red-coloured with non-aggregated gold nanoparticles when c>120μmol/L.The resolution limit of human eyes to this detection system was able to distinguish H2 O2 concentration difference of 20 μmol/L.However, the color distinction of solutions was unstable and has a quick change in 45 mins.The blue solution with c of 60 to 100μmol/L turned red gradually.The OD570 of solutions also demonstrates that the reaction was constantly changing in 3 h after detection.Moderate L-glutathione was added to terminate the reaction after 10 min to improve the detec-tion stability, which can stabilize the color distinction and effectively keep a constant OD570 .The spectra of the nan-oparticle dispersions show the absorption peak at 550 nm and there is a positive correlation between intensity and the H2 O2 concentration when c<100 μmol/L.The absorption peak shifted to 540 nm when c>120 μmol/L.By the spectral analysis, a linear relativity of regressive curves of OD630/545 and the concentration of H2 O2 was found. Then solutions with c from 100 to 120 μmol/L were detected and accurately quantified H2 O2 with concentration difference of 2 μmol/L to break the limit of detecting resolution.The detection system could provide a preferential platform for trace detection.