CAJ | 학술논문

目的：通过探讨雷帕霉素对人脐带来源间充质干细胞（UC-MSCs）衰老的影响，推动其临床应用。方法将人脐带间充质干细胞（MSCS）分为对照组及雷帕霉素组。β-半乳糖苷酶染色实验检测UC-MSC细胞内衰老相关β-半乳糖苷酶（SABG）的表达量；细胞计数试剂（CCK8）检测UC-MSC细胞的增殖；Real-time PCR检测UC-MSC细胞中衰老、增殖与干性相关 mRNA的表达。结果与无水乙醇对照组相比，雷帕霉素组细胞β-半乳糖苷酶染色阳性率及染色深度均降低；细胞增殖加快；增殖、干性基因升高同时伴有衰老相关基因的下降。结论雷帕霉素能够减缓人脐带MSCs的衰老。
목적：통과탐토뢰파매소대인제대래원간충질간세포（UC-MSCs）쇠로적영향，추동기림상응용。방법장인제대간충질간세포（MSCS）분위대조조급뢰파매소조。β-반유당감매염색실험검측UC-MSC세포내쇠로상관β-반유당감매（SABG）적표체량；세포계수시제（CCK8）검측UC-MSC세포적증식；Real-time PCR검측UC-MSC세포중쇠로、증식여간성상관 mRNA적표체。결과여무수을순대조조상비，뢰파매소조세포β-반유당감매염색양성솔급염색심도균강저；세포증식가쾌；증식、간성기인승고동시반유쇠로상관기인적하강。결론뢰파매소능구감완인제대MSCs적쇠로。
Objective To investigate the effect of Rapamycin in the senescence process of umbilical cord-derived mesenchymal stem cells (UC-MSCs).Methods UC-MSCs were divided into Rapamycin group and absolute alcohol control group.The measure of senescence-associated b-galactosidase(SABG) of UC-MSCs were detected by Beta galactosidase staining reagent.The proliferation performance of UC-MSC were examined by Cell Counting Kit-8(CCK8).The expressions of senescence,proliferation and stemness relative mRNA were examined by real-time PCR. Results Compared with the absolute alcohol control group,The Rapamycin group show a lower positive rate and dyeing depth of Beta galactosidase staining. Rapamycin treated UC-MSCs present a higher rate of proliferation.The expressions of proliferation,stemness related mRNA were elevated, with an decrease of senescence relative mRNA.Conclusion Rapamycin slows down the senescence phenotype.