浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2015年
8期
1268-1269
,共2页
结核病%抗结核抗体%结核感染T细胞斑点试验
結覈病%抗結覈抗體%結覈感染T細胞斑點試驗
결핵병%항결핵항체%결핵감염T세포반점시험
Tuberculosis%38kD-lgG%TSPOT-TB%Diagnosis
目的：探讨抗结核抗体（38kD-lgG）和结核感染T细胞斑点试验（TSPOT-TB）联合检测在结核病临床诊断中的价值。方法采用上述两种方法单项或联合检测疑结核患者114例，其中结核患者72例，非结核患者42例，对检测结果进行比较分析。结果 TSPOT-TB及38kD-IgG试验单项检测结核菌阳性（菌阳）结核患者的敏感性分别为90.63%和78.13%，二者差异有统计学意义（P<0.05），联合检测敏感性为96.88%。TSPOT-TB及38kD-IgG试验单项检测结核菌阴性（菌阴）结核患者的敏感性分别为80.00%和77.50%，联合检测敏感性为95.00%，联合检测与两种单项检测比较，差异有统计学意义（P<0.05）。对照组中，TSPOT-TB及38kD-IgG试验特异性分别为90.48%和78.57%，联合检测特异性为66.67%。TSPOT-TB试验对3种肺外结核的检测敏感性显著高于38kD-IgG试验（P<0.05）。结论对于菌阳患者，TSPOT-TB试验敏感性较高。对于菌阴患者，联合检测敏感性较高。38kD-lgG和TSPOT-TB联合检测可提高结核病的诊断敏感性。
目的：探討抗結覈抗體（38kD-lgG）和結覈感染T細胞斑點試驗（TSPOT-TB）聯閤檢測在結覈病臨床診斷中的價值。方法採用上述兩種方法單項或聯閤檢測疑結覈患者114例，其中結覈患者72例，非結覈患者42例，對檢測結果進行比較分析。結果 TSPOT-TB及38kD-IgG試驗單項檢測結覈菌暘性（菌暘）結覈患者的敏感性分彆為90.63%和78.13%，二者差異有統計學意義（P<0.05），聯閤檢測敏感性為96.88%。TSPOT-TB及38kD-IgG試驗單項檢測結覈菌陰性（菌陰）結覈患者的敏感性分彆為80.00%和77.50%，聯閤檢測敏感性為95.00%，聯閤檢測與兩種單項檢測比較，差異有統計學意義（P<0.05）。對照組中，TSPOT-TB及38kD-IgG試驗特異性分彆為90.48%和78.57%，聯閤檢測特異性為66.67%。TSPOT-TB試驗對3種肺外結覈的檢測敏感性顯著高于38kD-IgG試驗（P<0.05）。結論對于菌暘患者，TSPOT-TB試驗敏感性較高。對于菌陰患者，聯閤檢測敏感性較高。38kD-lgG和TSPOT-TB聯閤檢測可提高結覈病的診斷敏感性。
목적：탐토항결핵항체（38kD-lgG）화결핵감염T세포반점시험（TSPOT-TB）연합검측재결핵병림상진단중적개치。방법채용상술량충방법단항혹연합검측의결핵환자114례，기중결핵환자72례，비결핵환자42례，대검측결과진행비교분석。결과 TSPOT-TB급38kD-IgG시험단항검측결핵균양성（균양）결핵환자적민감성분별위90.63%화78.13%，이자차이유통계학의의（P<0.05），연합검측민감성위96.88%。TSPOT-TB급38kD-IgG시험단항검측결핵균음성（균음）결핵환자적민감성분별위80.00%화77.50%，연합검측민감성위95.00%，연합검측여량충단항검측비교，차이유통계학의의（P<0.05）。대조조중，TSPOT-TB급38kD-IgG시험특이성분별위90.48%화78.57%，연합검측특이성위66.67%。TSPOT-TB시험대3충폐외결핵적검측민감성현저고우38kD-IgG시험（P<0.05）。결론대우균양환자，TSPOT-TB시험민감성교고。대우균음환자，연합검측민감성교고。38kD-lgG화TSPOT-TB연합검측가제고결핵병적진단민감성。
ObjectiveTo evaluate the application value of tuberculosis antibody and enzyme linked immune-spot assay used for Clinical diagnosis of tuberculosis.MethodsThe two methods were used individually and in combination respectively to 72 active tuberculosis patients and 42 non-tuberculosis in control group.ResultsFor smear positive TB,the sensitivity of TSPOT-TB(90.63%)was significantly higher than that of 38kD-lgG test(78.13%)(P<0.05),and was epual to the sensitivity of 96.88% of combined detection;for bacteriological negative TB, the sensitivity of combined detection(95%)was significantly higher than that of 85% and 77.50% by TSPOT-TB and 38kD-lgG(P<0.05);42 non-tuberculosis control,the sensitivity were 78.57% and 90.48% respectively,the sensitivity of combined detection was 66.67%;the detection sensitivity of extra pulmonary TB by TSPOT-TB were significantly higher than those of 38kD-lgG,and was equal to the sensitivity of combined detection.ConclusionTSPOT-TB test in smear positive patients and tuberculous pleurisy and meningitis in patients with higher sensitivity,in the non tuberculosis population specificity is good;in the differential diagnosis of smear negative patients,38kD-lgG and TSPOT-TB test and application will improve the greater sensitivity.
