食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
14期
98-101
,共4页
张媛媛%张彬%韩爱云%张文娜%俞龙泉
張媛媛%張彬%韓愛雲%張文娜%俞龍泉
장원원%장빈%한애운%장문나%유룡천
高效液相色谱%蒸发光散射检测%低聚果糖
高效液相色譜%蒸髮光散射檢測%低聚果糖
고효액상색보%증발광산사검측%저취과당
high performance liquid chromatography%evaporative light scattering detection%fructooligosac鄄charides
通过对纸层析法,高效液相色谱-示差检测法和高效液相色谱-蒸发光散射检测法三种低聚果糖检测方法进行对比研究.结果发现,高效液相色谱-蒸发光散射检测法分离度高,重现性和准确性好,操作相对简单易行,优于其它两种方法.该方法采用氨基柱,流动相为乙腈与水,采用梯度洗脱,流速设定1.0 mL/min.ELSD的漂移管温度设定85℃;载气为空气,流速2.0 L/min.低聚果糖各组分的回收率范围为97.73%~99.39%,日内精密度<1.2%;日间精密度<2.8%.高效液相色谱-蒸发光散射检测法简便快捷,准确性,重现性好,更适合于低聚果糖的检测.
通過對紙層析法,高效液相色譜-示差檢測法和高效液相色譜-蒸髮光散射檢測法三種低聚果糖檢測方法進行對比研究.結果髮現,高效液相色譜-蒸髮光散射檢測法分離度高,重現性和準確性好,操作相對簡單易行,優于其它兩種方法.該方法採用氨基柱,流動相為乙腈與水,採用梯度洗脫,流速設定1.0 mL/min.ELSD的漂移管溫度設定85℃;載氣為空氣,流速2.0 L/min.低聚果糖各組分的迴收率範圍為97.73%~99.39%,日內精密度<1.2%;日間精密度<2.8%.高效液相色譜-蒸髮光散射檢測法簡便快捷,準確性,重現性好,更適閤于低聚果糖的檢測.
통과대지층석법,고효액상색보-시차검측법화고효액상색보-증발광산사검측법삼충저취과당검측방법진행대비연구.결과발현,고효액상색보-증발광산사검측법분리도고,중현성화준학성호,조작상대간단역행,우우기타량충방법.해방법채용안기주,류동상위을정여수,채용제도세탈,류속설정1.0 mL/min.ELSD적표이관온도설정85℃;재기위공기,류속2.0 L/min.저취과당각조분적회수솔범위위97.73%~99.39%,일내정밀도<1.2%;일간정밀도<2.8%.고효액상색보-증발광산사검측법간편쾌첩,준학성,중현성호,경괄합우저취과당적검측.
A High Performance Liquid Chromatography-Evaporative Light Scattering Detection method was compared with Paper Chromatography method and High Performance Liquid Chromatography-Refractive Index Detection method in this study. The comparison result showed that the High Performance Liquid Chromatography-Evaporative Light Scattering Detection method was significantly better than the other two methods. The experimental conditions were showed as follows: Compounds were successfully separated on a Waters NH2 column (4.6 mm) by a gradient elution. Acetonitrile and water were selected as the mobile phase A and B with a flow rate of 1.0 mL/min. The parameters of ELSD were set as follow:drift tube temperature was 85℃, air carrier gas flow rate was 2.0 L/min. The result showed that good separation effect was obtained and the recoveries were ranging from 97.73%to 99.39%. The intra-day precision (RSD) was less than 1.2%and the within-day precision (RSD) was less than 2.8 %. The method showed good separation effect, accuracy, reproducibility, and suitable for the detection of fructooligosaccharides in practice.