CAJ | 학술논문

对黄色蚕茧超声辅助酶法脱胶工艺进行研究。在单因素实验的基础上，选择对蚕茧脱胶率有显著影响的3个因素：p H （X1）、酶加量（X2）、超声功率（X3），进行三因素三水平的响应面分析实验。最终确定最优条件为：p H 5．5、酶加量5．5％（E／S）、超声功率390W、超声处理时间35min、温度60℃、料液比1∶30（m／v）。并且该最佳条件下脱胶率（37．62±0．34％）和脱下的丝胶蛋白DPPH自由基清除率（IC502．41±0．078 mg／mL ）、类胡萝卜素含量（0．34±0．038 m g／g ）和黄酮含量（0．59±0．062 m g／g ）均高于水浴煮沸脱胶和酶法脱胶，进一步表明了超声辅助酶法脱胶的优越性。
대황색잠충초성보조매법탈효공예진행연구。재단인소실험적기출상，선택대잠충탈효솔유현저영향적3개인소：p H （X1）、매가량（X2）、초성공솔（X3），진행삼인소삼수평적향응면분석실험。최종학정최우조건위：p H 5．5、매가량5．5％（E／S）、초성공솔390W、초성처리시간35min、온도60℃、료액비1∶30（m／v）。병차해최가조건하탈효솔（37．62±0．34％）화탈하적사효단백DPPH자유기청제솔（IC502．41±0．078 mg／mL ）、류호라복소함량（0．34±0．038 m g／g ）화황동함량（0．59±0．062 m g／g ）균고우수욕자비탈효화매법탈효，진일보표명료초성보조매법탈효적우월성。
The purpose of this research is to study the process of ultrasonic‐assisted enzymatic degumming of yellow cocoons .Based on the single factor tests ,the three factors :pH (X1 ) ,enzyme amount (X2 ) ,and the ratio of mate‐rial to solvent (X3 ) had a significant impact on degumming rate of natural yellow cocoons .Then the three factors were assessed by response surface methodology (RSM ) involving a three factors and three levels . The optimal process was determined as follows:the pH was 5 .5 ,the enzyme amount was 5 .5% (E/S) ,the ultrasonic power was 390 W ,the ultrasonic treatment time was 35 min ,the temperature was 60℃ ,and the ratio of material to sol‐vent was 1 ∶ 30(m/v) .Under such conditions ,the ultrasonic‐assisted enzymatic has higher degumming rate of 37 .62% ± 0 .34% ,and the sericin protein after ultrasonic‐assisted enzymatic degumming has higher DPPH scaven‐ging activity (IC50 2 .41 ± 0 .078mg/mL ) ,content of carotenoids (0 .34 ± 0 .038mg/g ) ,and flavonoids (0 .59 ± 0 .062mg/g) than boiling water bath and enzymatic degumming .The ultrasonic‐assisted enzymatic degumming was further demonstrated superior .