粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2015年
8期
62-65
,共4页
王丽娟%刘苏萌%李可%何培新
王麗娟%劉囌萌%李可%何培新
왕려연%류소맹%리가%하배신
黑苦荞%黄酮类化合物%稳定性%抑菌活性
黑苦蕎%黃酮類化閤物%穩定性%抑菌活性
흑고교%황동류화합물%은정성%억균활성
Black Tartary Buckwheat%flavoniods%stability%bacteriostasis activity
以黑苦荞为原料,研究黑苦荞中黄酮类化合物的稳定性和抑菌活性。结果表明:温度在20~80℃时黄酮类化合物含量基本不变;在弱酸性(pH 4~7)条件下黄酮类化合物的含量较为稳定;光照对黑苦荞黄酮的稳定性影响不大;加入氧化剂H2O2对黑苦荞黄酮的稳定性影响不大,而加入还原剂Na2SO3对其有强烈破坏作用;K+、Na+、Ca2+、Mg2+四种金属离子对苦荞黄酮稳定性无明显影响,Al3+,Fe2+、Fe3+对其影响较大,保存时应尽量避免与此类物质接触。抑菌实验表明,最小抑菌浓度(MIC)值分别为金黄色葡萄球菌25 mg/mL、枯草芽孢杆菌50 mg/mL、大肠杆菌100 mg/mL,黑苦荞黄酮类化合物对金黄色葡萄球菌、枯草芽孢杆菌、大肠肝菌均有较好的抑制作用。
以黑苦蕎為原料,研究黑苦蕎中黃酮類化閤物的穩定性和抑菌活性。結果錶明:溫度在20~80℃時黃酮類化閤物含量基本不變;在弱痠性(pH 4~7)條件下黃酮類化閤物的含量較為穩定;光照對黑苦蕎黃酮的穩定性影響不大;加入氧化劑H2O2對黑苦蕎黃酮的穩定性影響不大,而加入還原劑Na2SO3對其有彊烈破壞作用;K+、Na+、Ca2+、Mg2+四種金屬離子對苦蕎黃酮穩定性無明顯影響,Al3+,Fe2+、Fe3+對其影響較大,保存時應儘量避免與此類物質接觸。抑菌實驗錶明,最小抑菌濃度(MIC)值分彆為金黃色葡萄毬菌25 mg/mL、枯草芽孢桿菌50 mg/mL、大腸桿菌100 mg/mL,黑苦蕎黃酮類化閤物對金黃色葡萄毬菌、枯草芽孢桿菌、大腸肝菌均有較好的抑製作用。
이흑고교위원료,연구흑고교중황동류화합물적은정성화억균활성。결과표명:온도재20~80℃시황동류화합물함량기본불변;재약산성(pH 4~7)조건하황동류화합물적함량교위은정;광조대흑고교황동적은정성영향불대;가입양화제H2O2대흑고교황동적은정성영향불대,이가입환원제Na2SO3대기유강렬파배작용;K+、Na+、Ca2+、Mg2+사충금속리자대고교황동은정성무명현영향,Al3+,Fe2+、Fe3+대기영향교대,보존시응진량피면여차류물질접촉。억균실험표명,최소억균농도(MIC)치분별위금황색포도구균25 mg/mL、고초아포간균50 mg/mL、대장간균100 mg/mL,흑고교황동류화합물대금황색포도구균、고초아포간균、대장간균균유교호적억제작용。
The research wascarriedon stability and bacteriostasis activityof flavonoids in Black Tartary Buckwheat. The results showed that flavonoidscontent was essentially the same under the temperature at 20℃ to 80℃ ; The flavonoids were stable in slightly acidicconditions(pH 4~7); The light and theoxidant H2O2had noobvious influence,while the reducing agent Na2SO3 had a strongly destructive effecton it. The metal ions such as K+,Na+,Ca2+,Mg2+ had noobvious effecton the stabilityof black tartary buckwheat flavonoids. While Al3+,Fe2+,Fe3+had larger effect. The bacteriostatic action showed that MIC value respectively wereStaphylococcus aureus 25 mg/mL、Bacillus subtilis 50 mg/mL、Escherichiacoli 100 mg/mL,and flavonoids revealed excellent inhibitory effectsonStaphylococcus aureus、Bacillus subtilis and Escherichiacoli.