食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
14期
75-78
,共4页
豆粕%发酵%染料木素%培养条件
豆粕%髮酵%染料木素%培養條件
두박%발효%염료목소%배양조건
defatted soybean meal%fermentation genistein%fermentation conditions
通过单因素和正交试验确定, 得出纳豆芽孢杆菌A-3发酵豆粕产染料木素的最佳条件为8%豆粕添加量,培养温度为34℃,转速为180 r/min,培养64 h,pH为7.3,装液量为50 mL/250 mL,接菌量为10%.最终目的产物染料木素的产率为0.12%,转化率为89.13%,达到理想水平.
通過單因素和正交試驗確定, 得齣納豆芽孢桿菌A-3髮酵豆粕產染料木素的最佳條件為8%豆粕添加量,培養溫度為34℃,轉速為180 r/min,培養64 h,pH為7.3,裝液量為50 mL/250 mL,接菌量為10%.最終目的產物染料木素的產率為0.12%,轉化率為89.13%,達到理想水平.
통과단인소화정교시험학정, 득출납두아포간균A-3발효두박산염료목소적최가조건위8%두박첨가량,배양온도위34℃,전속위180 r/min,배양64 h,pH위7.3,장액량위50 mL/250 mL,접균량위10%.최종목적산물염료목소적산솔위0.12%,전화솔위89.13%,체도이상수평.
The optimal conditions of producing genistein demonstrated by applying sigle factor and response surface methodology were that the concentration of soybean meal, rotation speed, fermentation hours the optimal pH were 8 %, 180 r/min, 64 h, 7.3, the liquid loading amount was 50 mL/250 mL, the bacteria amount was 10 %. The yield of genistein ratio arrived at 0.12 %, the conversion rate was 89.13 %, which achieves the optimal level.
